不同食味水稻品种中与氮代谢相关酶活性的比较研究

为了研究水稻食味品质与氮代谢相关酶之间的关系,以津川1号和津原45稻种为试材,进行种子萌发获得水稻幼苗,分别测定幼苗和种子中的可溶性蛋白和谷氨酸的含量及谷草转氨酶(GOT)、谷丙转氨酶(GPT)、谷氨酸脱氢酶(GDH)、谷氨酰胺合成酶(GS)和谷氨酸合成酶(GOGAT)的活性.结果表明,不同食味水稻品种之间幼苗和种子中的GOT、GPT活性和可溶性蛋白含量均无显著差异,而同一品种幼苗内的可溶性蛋白和谷氨酸的含量及GOT、GPT、GDH活性均显著高于种子;谷氨酸含量与GS/GDH的比值呈显著正相关,并与水稻食味品质呈正相关,其中津川1号幼苗的GS/GDH比值显著高于津原45,其谷氨酸含量显著高于津原45;不同食味水稻品种的硝酸还原酶活性不同.

Comparative study on activities of enzymes related to nitrogen metabolism in rice cultivars with different eating tastes

In order to study the relationship between rice eating quality and enzymes related to nitrogen metabolism, seeds of Jinchuan 1 and Jinyuan 45 were used in this research by germinating seeds and getting seedlings. The contents of soluble protein and glutamate, activities of GOT, GPT, GDH, GS and GOGAT were detected. The results showed, there was no significant difference on contents of soluble protein and activities of GOT, GPT from seeds and seedlings of rice cultivars with different eating tastes, but in the same cultivar, contents of soluble protein and glutamate, activities of GOT, GPT, GDH in seedling leaves were significantly higher than those in seeds; GS/GDH value in the seedlings of Jinchuan 1 was significantly higher than that in Jinyuan 45, but no difference was found in seeds. The content of glutamate in seedling leaves of Jinchuan 1 was significantly higher than that in Jinyuan 45, but no difference was found in seeds, the change trend of glutamate had a significantly positive correlation with the change of GS/GDH value and with the difference of rice eating tastes. Cultivars with different eating tastes showed different activity levels of nitrite reductase.