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| 茶叶中EGCG3″Me的分析方法研究 | |
| 引用本文： | 孙业良,吕海鹏,林智,黎星辉.茶叶中EGCG3″Me的分析方法研究[J].茶叶科学,2009,29(5). |
| 作者姓名： | 孙业良 吕海鹏 林智 黎星辉 |
| 作者单位： | 1. 南京农业大学茶叶研究所,江苏,南京,210095;中国农业科学院茶叶研究所/茶叶加工工程研究中心,浙江,杭州,310008 2. 中国农业科学院茶叶研究所/茶叶加工工程研究中心,浙江,杭州,310008 3. 南京农业大学茶叶研究所,江苏,南京,210095 |
| 基金项目： | 国家科技支撑计划，浙江省自然科学基金项目 |
| 摘 要： | 采用制备HPLC系统进行分离纯化并进一步结合NMR、LC/MS进行结构鉴定,从茶叶中分离纯化出了EGCG3″Me;研究了不同提取条件对茶叶中EGCG3″Me含量的影响,初步建立了用反相高效液相色谱测定茶叶中EGCG3″Me含量的分析方法。结果表明,该方法重现性好,适用于常规定量分析,平均加样回收率为95.48%,RSD为3.31%;采用蒸馏水在90℃的水浴浸提5min,茶汤中EGCG3"Me的浓度可以达到最高值。 |
| 关 键 词： | 茶 EGCG3"Me 分析 含量 |
Study on the Analytical Method of EGCG 3″Me by HPLC in Tea |
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| SUN Ye-liang,LU Hai-peng,LIN Zhi,LI Xing-hui.Study on the Analytical Method of EGCG 3″Me by HPLC in Tea[J].Journal of Tea Science,2009,29(5). | |
| Authors: | SUN Ye-liang LU Hai-peng LIN Zhi LI Xing-hui |
| Abstract: | EGCG3″Me was isolated by preparative HPLC system from tea and identified by NMR and LC/MS. The influence of different extracting method on the EGCG3″Me content in tea was discussed, and a simple high performance liquid chromatography analytical method of EGCG3″Me was developed. Results showed that the analytical method showed a good linear relationship between the peak area and content of EGCG3″Me (R2=0.9998) and high reproducibility. The average recovery rate of EGCG3″Me was 95.48% and the relative standard deviation(RSD)was 3.31%. The EGCG3″Me concentration in tea infusion can reach highest when the tea sample was immersed in distilled water for 5 min at 90℃. |
| Keywords: | EGCG3"Me |
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