| 彩椒的离体快繁研究 |
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| 引用本文： | 吴 蓓,周 娟,戴修纯,邹集文,刘绍钦.彩椒的离体快繁研究[J].热带作物学报,2013,33(3):1-3. |
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| 作者姓名： | 吴 蓓 周 娟 戴修纯 邹集文 刘绍钦 |
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| 作者单位： | 广东省广州市农业科学研究院 广东广州 510308;广东省广州市农业科学研究院 广东广州 510308;广东省广州市农业科学研究院 广东广州 510308;广东省广州市农业科学研究院 广东广州 510308;广东省广州市农业科学研究院 广东广州 510308 |
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| 摘 要： | 以红盾彩椒为试材，进行组培快繁，研究不同激素类型与配比组合对愈伤组织诱导、不定芽分化及芽的伸长的影响，并初步建立彩椒的组培快繁体系。 结果表明：子叶愈伤诱导最适培养基为：MS＋0.3 mg/L 6-BA＋1.0 mg/L NAA；不定芽诱导最适培养基为：MS＋3.0 mg/L 6-BA＋0.5 mg/L IAA＋4.0 mg/L AgNO3；芽伸长最适培养基为：MS＋2.0 mg/L 6-BA＋0.2 mg/L IAA＋2.0 mg/L ZT＋2.0 mg/L GA3；最后在1/2MS＋0.5 mg/L IBA(或 0.5 mg/L NAA)培养基上进行生根培养。
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| 关 键 词： | 彩椒 离体快繁 组培 |
In vitro Propagation of Sweet Pepper(Capsicum annuum L.) |
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| Institution: | Guangzhou Academy of Agricultural Sciences, Guangzhou, Guangdong 510308, China;Guangzhou Academy of Agricultural Sciences, Guangzhou, Guangdong 510308, China;Guangzhou Academy of Agricultural Sciences, Guangzhou, Guangdong 510308, China;Guangzhou Academy of Agricultural Sciences, Guangzhou, Guangdong 510308, China;Guangzhou Academy of Agricultural Sciences, Guangzhou, Guangdong 510308, China |
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| Abstract: | The sweet pepper (Capsicum annuum L.) were used for tissue culture to establish a high-frequency regeneration system, and the effects of different hormone combinations and culture medium were groped on the callus induction, adventitious bud differentiation and elongation. The results showed that the appropriate induction medium for the formation of callus from cotyledons was MS+0.3 mg/L 6-BA+ 1.0 mg/L NAA; the appropriate medium for the adventitious buds formation was MS+ 3.0 mg/L 6-BA + 0.5 mg/L IAA + 4.0 mg/L AgNO3; the appropriate elongation and radication media were MS+2.0 mg/L 6-BA + 0.2 mg/L IAA + 2.0 mg/L ZT +2.0 mg/L GA3 and 1/2MS+0.5 mg/L IBA (or 1/2MS+ 0.5 mg/L NAA) . |
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| Keywords: | Sweet pepper (Capsicum annuum L ) rapid propagation in vitro |
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