| 稻瘟病菌拮抗微生物的筛选及鉴定 |
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| 引用本文： | 游春平,肖爱萍,李湘明,唐祥宁. 稻瘟病菌拮抗微生物的筛选及鉴定[J]. 江西农业大学学报, 2001, 23(4): 519-521 |
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| 作者姓名： | 游春平 肖爱萍 李湘明 唐祥宁 |
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| 作者单位： | 江西农业大学农学院,江西南昌,330045;江西农业科学院,江西莲塘,330200 |
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| 基金项目： | 江西省自然科学基金 (9930 0 12 )和 (0 130 0 2 7),江西省重点项目 (2 0 0 110 10 0 10 9)资助项目 |
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| 摘 要： | 将稻叶和种子浸在NB培养液中,并研碎成浆浆液,用接种环挑少量匀浆液在NB琼脂平板上划线分离,获得356个菌株,分别将它们接种于NB培养液中,振荡培养3d,并利用它们的培养液对稻瘟菌分布孢子进行萌发抑制试验,最终获得具显抑制作用（分生孢子萌发抑制率分别为82．5％和100％）的2个拮抗菌株,按照伯杰手册进行严格的鉴定,初步确定为枯草芽孢杆菌（Bacillus subtilis) 和短小芽孢杆菌（B.pumillus)。
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| 关 键 词： | 稻瘟病菌 水稻 拮抗微生物 |
| 文章编号： | 1000-2286(2001)04-0519-03 |
| 修稿时间： | 2001-09-08 |
Screening and Identification of Antagonistic Microorganisms Against Pyricularia grisea |
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| YOU Chun-ping ,XIAO Ai-ping ,LI Xiang-ming ,TANG Xiang-ning. Screening and Identification of Antagonistic Microorganisms Against Pyricularia grisea[J]. Acta Agriculturae Universitis Jiangxiensis, 2001, 23(4): 519-521 |
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| Authors: | YOU Chun-ping XIAO Ai-ping LI Xiang-ming TANG Xiang-ning |
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| Affiliation: | YOU Chun-ping 1,XIAO Ai-ping 1,LI Xiang-ming 2,TANG Xiang-ning 1 |
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| Abstract: | The leaves or seeds of rice were immerged in NA cultural solution and ground. The supernatant was lined on NB agar and cultured for 12 h, 356 single-clone isolates were obtained from the cultures. After these isolates were cultured in NB cultural solution for 3d, their supernatant was used for test of antagonism against Pyricularia grisea. Two significantly antagonistic isolates were gained (their inhibitive rate against germination of conidia of P.grisea being 82.5% and 100%, respectively), and identified as Bacillus subtilis and B.pumillus according to Bergey's manual of systematic bacteriology. |
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| Keywords: | Pyricularia grisea rice antagonistic microorganisms |
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