直接蒸馏法快速测定饲料蛋白质含量的研究

利用直接蒸馏法（direct distillation）原理，经研究改进而建立的快速测定玉米、豆粕、菜籽饼、鱼粉、麸皮、猪配合饲料、鸡配合饲料蛋白质含量的新方法。3个实验室对7种类型142个饲料产品的研究结果表明，本法与国标法（凯氏法）有很好的相关性，两法测定值相对偏差均达到国家标准规定的要求。相关系数r=0.9745～0.9932，实验室间变异系数 < 3%,实验室内变异系数 < 2%。样品不需消化过程，20min可提供粗蛋白质含量数据，比国标法具有快速、能耗低、节约试剂、减少污染和操作简便等优点。

The Study on the Quick Determination of Protein Content in Feedstuffs by Direct Distillation Method

By using the direct distillation ( DD ) principle , an improved and quick method which can determine the protein content in maize , soybean meal , rapeseed meal , fish meal , wheat bran , formula feed for swine and formula feed for chicken was estab- lished. The result of the study on 142 products belonging to 7 kinds of feedstuffs in three laboratories suggested that , the DD method has fine correlation with the National Standard Method (Kjeldahl Method ) . The relative deviation of the two methods was in conform to the National Standard. The coefficient of variation between different labora- toies was lower than 3% , the coefficient of variation within single laboratory was lower than 2%. There was no process of digestion in DD method and the data of the result were given in 20 min. Compared with the National Standard Method , the DD method has many advantages such as quiclcness , low energy consumption , agent saving , pollution reducing and simple Operation etc.,