| 苦瓜种子蛋白质组双向电泳技术条件的优化 |
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| 引用本文： | 黄玉辉,黄如葵,梁家作,黄熊娟,冯诚诚,陈小凤,刘杏连,秦健. 苦瓜种子蛋白质组双向电泳技术条件的优化[J]. 种子, 2017, 0(10): 42-45. DOI: 10.16590/j.cnki.1001-4705.2017.10.042 |
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| 作者姓名： | 黄玉辉 黄如葵 梁家作 黄熊娟 冯诚诚 陈小凤 刘杏连 秦健 |
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| 作者单位： | 1. 广西农业科学院蔬菜研究所,南宁530007;广西作物遗传改良生物技术重点实验室,南宁530007;2. 广西农业科学院蔬菜研究所,南宁,530007 |
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| 基金项目： | 广西自然科学基金(2013 GXNSFDA 019010),现代农业产业技术体系建设专项资金(CARS-25),广西农业科学院基本科研业务专项(2015 YT 66),广西农业科学院基本科研业务专项(2015 YZ 01),广西农业科学院基本科研业务专项(桂农科2016 YM 24) |
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| 摘 要： | 通过对苦瓜种子蛋白质样品的提取方法、等电聚焦参数、SDS-PAGE分离胶浓度以及胶条pH范围的优化筛选,建立了适合苦瓜种子蛋白组分析的双向电泳体系.结果表明:使用TCA-丙酮提取方法提取苦瓜种子蛋白,更适用于双向电泳分析,结合使用pH=5~8 IPG胶条以及优化的聚焦程序,能获得分辨率较高、蛋白点清晰、重复性好的2-DE图谱.经银染显色,可检测约660个蛋白点,主要分布在p H=5~8;该体系适合用于分析苦瓜种子的蛋白质组.
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| 关 键 词： | 苦瓜种子 蛋白质组 双向电泳 系统优化 |
Optimiztion of Two -dimensional Gel Electrophoresis for Proteome of M omordica charantia Seed |
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| HUANG Yuhui,HUANG Rukui,LIANG Jiazuo,HUANG Xiongjuan,FENG Chengcheng,CHEN Xiaofeng,LIU Xinglian,QIN Jian. Optimiztion of Two -dimensional Gel Electrophoresis for Proteome of M omordica charantia Seed[J]. Seed, 2017, 0(10): 42-45. DOI: 10.16590/j.cnki.1001-4705.2017.10.042 |
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| Authors: | HUANG Yuhui HUANG Rukui LIANG Jiazuo HUANG Xiongjuan FENG Chengcheng CHEN Xiaofeng LIU Xinglian QIN Jian |
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| Abstract: | Through optimization and screening of two-dimensional electrophoresis sample preparation methods of seeds protein of Momordica charantia ,the procedure of isoelectric focus ,concentration of the second-dimensional SDS separation gel and the pH range of solidifying strips ,electrophoresis sys-tem that was suitable to seed protein of Momordica charantia was structured .The results showed that highly pure seed protein can be obtained with trichloroacetic acid (TCA)-acetone method ,2-DE images of protein of high resolution ratio and good repetitiveness can be got with optimized isoelectric focus procedure .About 660 clear protein spots were detected by two dimensional electrophoresis (2-DE) and silver staining with isoelectric points at pH 5-8 . |
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| Keywords: | momordica charantia seed proteome two-dimensional electrophoresis(2-DE) system optimization |
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