| 离体条件下影响梨叶片再生苗生根的因素研究 |
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| 引用本文： | 徐凌飞,魏鸫湘,杨华江,刘鲜艳,李嘉瑞. 离体条件下影响梨叶片再生苗生根的因素研究[J]. 西北农业学报, 2007, 16(3): 109-111 |
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| 作者姓名： | 徐凌飞 魏鸫湘 杨华江 刘鲜艳 李嘉瑞 |
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| 作者单位： | 1. 西北农林科技大学园艺学院,陕西杨凌,712100
2. 延安市洛川农科所,陕西洛川,727400 |
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| 基金项目： | 西北农林科技大学校科研和教改项目;遗传育种专项基金 |
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| 摘 要： | 利用正交设计研究了不同基本培养基、不同浓度的吲哚丁酸、萘乙酸、蔗糖以及暗培养时间等对砀山酥梨叶片再生苗生根的影响。结果表明,5个因子对叶片再生苗生根的影响程度依次为基本培养基、吲哚丁酸、蔗糖、萘乙酸和暗培养时间,其中不同的基本培养基、不同浓度的吲哚丁酸和蔗糖对梨叶片再生苗的生根有极显著影响。砀山酥梨叶片再生苗生根的适宜组合为1/4MS 0.5 mg.L-1IBA Sucrose 20 g.L-1,暗培养5 d。
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| 关 键 词： | 梨 叶片再生苗 正交试验 生根 |
| 文章编号： | 1004-1389（2007）03-0109-03 |
| 收稿时间： | 2006-09-05 |
| 修稿时间： | 2006-09-052006-12-06 |
Effect of Some Factors on Rooting of the Regenerated Plants from Pear Leaf in vitro |
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| XU Ling-fei,WEI Dong-xiang,YANG Hua-jiang,LIU Xian-yan and LI Jia-rui. Effect of Some Factors on Rooting of the Regenerated Plants from Pear Leaf in vitro[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2007, 16(3): 109-111 |
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| Authors: | XU Ling-fei WEI Dong-xiang YANG Hua-jiang LIU Xian-yan LI Jia-rui |
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| Affiliation: | College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China;Luochuan Agriculture Science Research Institute, Luochuan Shaanxi 727400, China;College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China;College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China;College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China |
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| Abstract: | The different basal medium,levers of IBA,NAA,sucrose and the dark culture periods were used to compared their effects on rooting of the regenerated plant from leafs of pear cultvars Dangshansu by using the orthogonal experiment design.The results showed that the effective order of five factors on rooting of the regenerated plant was basal medium,IBA,sucrose,NAA and the dark culture periods.The different basal medium,IBA,and sucrose had significant effect on rooting of the regenerated plant.The best combination for Dangshansu pear was 1/4MS+IBA0.5 mg·L-1 +sucrose 20 g·L-1,and dark cultured for 5 d. |
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| Keywords: | Pear Regenerated plants of the leafs Orthogonal experiment Rooting |
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