| 不同激素对菜用大黄茎尖离体再生的影响研究 |
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| 引用本文： | 蔡祖国,周岩,卢莉,赵一鹏.不同激素对菜用大黄茎尖离体再生的影响研究[J].广东农业科学,2010,37(3):132-134,144. |
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| 作者姓名： | 蔡祖国 周岩 卢莉 赵一鹏 |
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| 作者单位： | 1. 河南科技学院园林学院,河南,新乡,453003
2. 河南科技学院生命科技学院,河南,新乡,453003 |
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| 基金项目： | 河南科技学院科研启动基金(050103);;教育部归国留学人员科研启动基金(2005);;河南省教育厅创新人才基金(2007KYCX016);;河南省杰出青年基金(074100510019) |
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| 摘 要： | 以菜用大黄无菌苗的茎尖为外植体,探讨了不同激素对菜用大黄茎尖离体再生能力的影响。结果显示,不同激素对菜用大黄不定芽再生有显著影响。6-BA对菜用大黄茎尖增殖的效果优于KT,MS+6-BA1.0mg/L+NAA1.0mg/L培养基可获得最大增殖系数(3.1);新生不定芽经生根培养后可获得完整植株,而1/2MS+NAA0.5mg/L的生根培养基生根率高达92.6%。再生植株经驯化炼苗后,可获得75%的移栽成活率。
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| 关 键 词： | 菜用大黄 植物激素 组织培养 增殖 生根 |
Effects of different hormone on the regeneration of shoot-tip of culinary rhubarb (Rheum rhaponticum L.) in vitro |
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| CAI Zu-guo,ZHOU Yan,LU Li,ZHAO Yi-peng.Effects of different hormone on the regeneration of shoot-tip of culinary rhubarb (Rheum rhaponticum L.) in vitro[J].Guangdong Agricultural Sciences,2010,37(3):132-134,144. |
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| Authors: | CAI Zu-guo ZHOU Yan LU Li ZHAO Yi-peng |
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| Institution: | 1.School of Horticulture and Landscape Architecture;Henan Institute of Science and Technology;Xinxiang 453003;China;2. School of Life Science and Technology;China |
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| Abstract: | Effects of plant hormone on regeneration of adventitious buds and establishment of micropropagation of culinary rhubarb (Rheum rhaponticum L.) were studied using shoot-tips of in vitro seedling as explants. The results showed that the plant growth regulators played significant roles on regeneration of adventitious buds. 6-BA had better effect than KT in initiation and multiplication of adventitious buds, showing the highest multiplication rate (3.1) on medium of MS+6-BA 1.0 mg/L+NAA1.0 mg/ L. Rooting medium... |
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| Keywords: | rhubarb plant hormone tissue culture multiplication rooting |
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