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Root and crown rot of strawberry caused by Pythium helicoides and its distribution in strawberry production areas of Japan |
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| Authors: | Yasushi Ishiguro Kayoko Otsubo Hideki Watanabe Mikihiko Suzuki Kiichi Nakayama Takashi Fukuda Masashi Fujinaga Haruhisa Suga Koji Kageyama |
| Affiliation: | 1. River Basin Research Center, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan 2. Gifu Prefectural Agricultural Technology Center, 729 Matamaru, Gifu, 501-1152, Japan 3. Shizuoka Prefectural Research Institute of Agriculture and Forestry, 678-1 Tomioka, Iwata, 438-0803, Japan 4. Tochigi Prefectural Agricultural Experiment Station, 1080 Kawaraya-cho, Utsunomiya, 320-0002, Japan 5. Nagano Vegetable and Ornament Crops Experiment Station, 1066-1 So-ga Tokoo, Shiojiri, 399-6461, Japan 6. Life Science Research Center, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan |
| Abstract: | Pythium species were isolated from seedlings of strawberry with root and crown rot. The isolates were identified as P. helicoides on the basis of morphological characteristics and sequences of the ribosomal DNA internal transcribed spacer regions. In pathogenicity tests, the isolates caused root and crown rot similar to the original disease symptoms. Multiplex PCR was used to survey pathogen occurrence in strawberry production areas of Japan. Pythium helicoides was detected in 11 of 82 fields. The pathogen is distributed over six prefectures. |
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