芜菁、萝卜和大头菜块根品质及营养价值比较研究

目的优化蔓菁游离小孢子培养体系，提高出胚率，为蔓菁品种的纯化和种质创新提供重要基础。方法将54个蔓菁品种小孢子低温(4 ℃)和高温(33 ℃)各处理24 h，从中筛选出1个出胚率较高的品种，通过调整活性炭—琼脂糖及植物激素配比优化其小孢子培养体系。结果蔓菁品种KTRG-B-15的小孢子出胚数最多，为25胚/30蕾；高质量浓度琼脂糖可以抑制小孢子成胚；添加0.1 mg/L NAA和0.2 mg/L 6-BA时出胚数最多，为137胚/30蕾。结论小孢子培养体系优化有效提高了蔓菁小孢子的出胚数，优化后的出胚效率是优化前的5.48倍。

Immunomodulatory activity of sulforaphane,a naturally occurring isothiocyanate from broccoli (Brassica oleracea)

PurposeOptimizing the microspore culture system in turnip to improve the embryo yield, providing an important basis for the purification of turnip varieties and germplasm innovation. MethodAfter treatments at low temperature (4 ℃) and subsequent high temperature (33 ℃) for 24 hours respectively, one variety of turnip with a higher embryo emergence rate were screened from 54 varieties of turnip, and the microspore culture system of turnip was optimized by adjusting the ratio of activated carbon-agarose and the ratio of plant hormones. ResultsMicrospore embryogenesis of the variety KTRG-B-15 was the highest (25 embryos/30 buds). High mass concentrations of agarose could inhibit microspore embryogenesis. Microspore embryogenesis was ashigh as 137 embryos/30 buds after 0.1 mg/L NAA and 0.2 mg/L 6-BA were used in culture medium. ConclusionThe optimization of microspore culture system effectively improves the number of microspore embryogenesis in turnip, which is 5.48 times higher than that before optimization.