Detection of Water Buffalo Sex Chromosomes in Spermatozoa by Fluorescence in situ Hybridization |
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| Authors: | T Révay A Kovács GA Presicce W Rens I Gustavsson |
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| Institution: | Budapest University of Technology and Economics, Department of Biochemistry and Food Technology, Budapest, Megyetem rkp. 3. Hungary;;Research Institute for Animal Breeding and Nutrition, Herceghalom, Gesztenyés u. 1., Hungary;;ARSIAL, Centro Sperimentale per la Zootecnia, Via R. Lanciani, Roma, Italy;;University of Cambridge, Department of Clinical Veterinary Medicine, Madingley Road, Cambridge, UK and;Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics, Centre of Reproductive Biology in Uppsala, Uppsala, Sweden |
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| Abstract: | In order to identify X‐ and Y‐bearing spermatozoa in water buffalo by fluorescence in situ hybridization (FISH), some available probes of closely related species were examined. An X‐ and Y‐specific probe set, made from flow sorted yak chromosomes, labelled in somatic metaphases of water buffalo the whole X and Y, respectively, except their centromere regions. A cattle Y‐chromosome repeat sequence (BC1.2) showed strong signal on the telomere region of the buffalo Y‐chromosome, demonstrating the evolutionary conservation of this locus in water buffalo. In hybridization experiments with spermatozoa from five buffaloes, the yak X‐Y paint set demonstrated clear signals in more than 92% (46.8% X and 45.8% Y) of the cells. Using the cattle Y‐chromosome specific BC1.2 probe, clear hybridization signal was detected in more than 48% of the cells. Statistical analysis showed that there was no significant difference between bulls or from the expected 50 : 50 ratio of X‐ and Y‐bearing cells. The probes presented here are reliable to assess separation of X‐ and Y‐bearing spermatozoa. |
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