马棘种子发芽及人工加速老化测定标准化研究

以水土保持植物马棘Indigofera pseudotinctoria种子为研究对象,针对其质量检测存在的问题,确定适宜的发芽和加速老化条件。在发芽试验中,设置15~25、15~30、15~35、20~30、20~35、25~35、20、25、30、35℃共10种温度处理,光照设8 h光照和24 h黑暗2个处理;在加速老化试验中,设置39、41、43、45℃共4个温度水平和24~96 h之间的7个时间处理。研究结果表明:不同光照和温度条件下马棘种子发芽率不同,在8 h光照和24 h黑暗适宜发芽的条件均为恒温25℃或变温20~30℃,种子发芽对光照不敏感,但黑暗条件会导致种苗黄化,温度和光照间存在互作效应。初次发芽计数时间为7 d,末次发芽计数时间为14d。加速老化最适条件为43℃、72 h,且老化温度和时间存在互作效应。

Standardization of germination and accelerated aging testing of the Indigofera pseudotinctoria seeds

In order to standardize the testing method of germination of Indigofera pseudotinctoria seed, an experiment was conducted to determine the optimum condition for seed germination and accelerated aging test. In the germination test, the temperature treatments were adopted including 15-25 ℃, 15-30 ℃, 15-35 ℃, 20-30 ℃, 20-35 ℃, 25-35 ℃, 20 ℃, 25 ℃, 30 ℃ and 35 ℃, 2 light treatments(8 h lightness+16 darkness and 24 h darkness). In the accelerated aging trial, ageing temperature were 39 ℃, 41 ℃, 43 ℃, 45 ℃ and ageing duration were 24 h, 36 h, 48 h, 60 h, 72 h, 84 h, 96 h. The results showed that different combination of light and temperature treatments influenced germination rates of I. pseudotinctoria seed. Optimum conditions for seed testing of germination was constant temperature 25 ℃ or alternate temperature 20-30 ℃, 8 h light will be helpful for seedling growth, while total dark condition leads to the etiolated seedlings. There was an interaction effect between temperature and lightness. The first count time is the 7 th day after seed germination, and the last time is the 14 th day for the seedling evaluation. The optimal temperature of accelerated aging treatment was 43 ℃ and the ageing duration was 72 h and there was an interaction effect between accelerated aging temperature and duration.