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| SRAP/RSAP标记鉴定印度南瓜种子纯度的方法 | |
| 引用本文： | 刘泽发,孙小武,董亚静,罗育才,曾永贤,邹 坤.SRAP/RSAP标记鉴定印度南瓜种子纯度的方法[J].西北农业学报,2011,20(4):124-128. |
| 作者姓名： | 刘泽发 孙小武 董亚静 罗育才 曾永贤 邹 坤 |
| 作者单位： | 1. 湖南人文科技学院,湖南娄底,417000 2. 湖南农业大学园艺园林学院,长沙,410128 3. 湖南湘研种业有限公司,长沙,410000 |
| 摘 要： | 应用相关序列扩增多态性(SRAP)标记和限制性位点扩增多态性(RSAP)标记对印度南瓜品种杂交种子纯度进行鉴定试验。结果表明,筛选出的引物E1M5和R1R7可很好地区分出红栗二号、红栗、锦栗二号和锦栗南瓜,利用RSAP分子标记R1R7可检测出锦栗杂交种子纯度,分子标记检测结果与田间检测结果吻合率达到90%。 |
| 关 键 词： | 印度南瓜 RSAP SRAP 纯度鉴定 |
Hybrid Purity Detection of Pumpkin (C.maxima L.) Varieties with SRAP and RSAP Markers |
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| LIU Zef,SUN Xiaowu,DONG Yajing,LUO Yucai,ZENG Yongxian and ZOU Kun.Hybrid Purity Detection of Pumpkin (C.maxima L.) Varieties with SRAP and RSAP Markers[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(4):124-128. | |
| Authors: | LIU Zef SUN Xiaowu DONG Yajing LUO Yucai ZENG Yongxian and ZOU Kun |
| Institution: | LIU Zefa1,SUN Xiaowu2,DONG Yajing3,LUO Yucai1,ZENG Yongxian1 and ZOU Kun1(1.Hunan University of Humanities,Science and Technology,Loudi Hunan 417000,China,2.Landiscape and Horticultural Institute of Hunan Agriculture University,Changsha 410128,3.Hunan Xiangyan Seed Co.,LTD.,Changsha 410000,China) |
| Abstract: | The sequence-related amplified polymorphism(SRAP) markers and the restriction site amplification polymorphism(RSAP) markers were applied to test hybrid seed purity of squash(Cucurbit Maxima L.).The results showed that E1M5 and R1R7 marker can distinguish well Hongli No.2,Hongli,Jinli No.2 and Jinli variety.RSAP marker R1R7 can test Jinli hybrid seed purity successfully,with a coincidence rate of 90% to the field test results. |
| Keywords: | RSAP SRAP |
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