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| 展示狂犬病病毒保护性抗原的重组犬2型腺病毒的构建 | |
| 引用本文： | 王燕,张守峰,崔艳,徐振波,于恒智,扈荣良,张乐萃.展示狂犬病病毒保护性抗原的重组犬2型腺病毒的构建[J].中国动物检疫,2008,25(2):24-26. |
| 作者姓名： | 王燕 张守峰 崔艳 徐振波 于恒智 扈荣良 张乐萃 |
| 作者单位： | 1. 青岛农业大学动物科技学院,青岛,266109;军事医学科学院军事兽医研究所,长春,130062 2. 军事医学科学院军事兽医研究所,长春,130062 3. 青岛农业大学动物科技学院,青岛,266109 |
| 基金项目： | 国家自然科科基金（30571379） |
| 摘 要： | 本试验以犬2型腺病毒全基因组重组质粒pPolyⅡ-CAV-2为基础,以8202株狂犬病病毒基因组为模板,通过PCR扩增得到狂犬病病毒糖蛋白膜外区基因(8202-G″)。通过MluⅠ和EcoRⅠ双酶切pPolyⅡ-CAV-2质粒,获得含有Ⅸ蛋白的340bp片段,将其克隆入pEGFP-C1-dAseⅠ质粒中,构建pEGFP-SIX载体,该载体经AseⅠ单酶切插入8202-G″,即在编码Ⅸ蛋白基因的最后密码子与终止密码子之间按与Ⅸ蛋白编码链相同转录方向插入8202-G″基因,获得重组基因组质粒和pPolyII-CAV-2-ⅨG(34.8kb)。酶切鉴定结果显示,目的基因均克隆进Ⅸ蛋白中。 |
| 关 键 词： | 狂犬病 糖蛋白 犬2型腺病毒 重组病毒 |
| 文章编号： | 1005-944X（2008）02-0025-03 |
Construction of a recombinant canine adenovirus displaying the protective antigen of rabies virus |
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| Wang Yan,Zhang Shoufeng,Cui Yan,Xu Zhenbo,Yu Hengzhi,Hu Rongliang,Zhang Lecui.Construction of a recombinant canine adenovirus displaying the protective antigen of rabies virus[J].China Journal Of Animal Quarantine,2008,25(2):24-26. | |
| Authors: | Wang Yan Zhang Shoufeng Cui Yan Xu Zhenbo Yu Hengzhi Hu Rongliang Zhang Lecui |
| Institution: | 1)(1.College of veterinary and animal science, Qingdao Agricultural University, Qing dao 266109, China;2. Military Veterinary Institute, Academy of Military Medical Sciences, Chang chun 130062, China) |
| Abstract: | An recombinant genome of canine adenovirus type-2 was constructed in this experiment. The external part of glycoprotein was amplified through RT-PCR and cloned into Ase I site of pEGFP-C 1-dAse I containing the capsid protein IX of canine adenovirus type-2, pEGFP-SIX was obtained. The capsid protein IX of CAV-2 genome was replaced with the recombinant protein IX that was released by Ase I from pEGFP-SIX rightward between the last codon of the IX gene and the stop codon. As a result, the recombinant genome of CAV-2 was obtained. The results showed that the target genes were cloned into the IX protein by restriction enzyme digestion. |
| Keywords: | Rabies virus Glycoprotein Canine adenovirus type-2 Recombinant virus |
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