| 水稻CAT与逆境应答关系及酶活性分析 |
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| 引用本文： | 李耀文,柳参奎. 水稻CAT与逆境应答关系及酶活性分析[J]. 广西农业生物科学, 2009, 0(3): 509-514 |
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| 作者姓名： | 李耀文 柳参奎 |
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| 作者单位： | 东北林业大学盐碱地生物资源环境研究中心抗性分子生物学实验室; |
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| 基金项目： | 国家林业局948项目(2008-4-29);;黑龙江省杰出青年科学基金(JC200609)共同资助 |
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| 摘 要： | 通过氧化氢酶（catalase，CAT）是一种四聚体血红素酶，主要存在于过氧化物酶体与乙醛酸循环体及相关细胞区域内。水稻中含有CAT1、CAT2和CAT3三种主要同工酶。它们在各组织不同的发育阶段，发挥着重要作用。在本研究解析了水稻过氧化氢酶同工酶基因在NaCl、NaHCO3、Na2CO3、PEG6000、H2O2、低温等逆境处理下mRNA表达特性。结果表明在不同的逆境下过氧化氢酶同工酶在表达上存在差异性。同时利用蛋白表达系统，对水稻过氧化氢酶CAT1和CAT3蛋白进行了纯化实验，对纯化的蛋白的酶活性分析的结果表明，CAT1和CAT3的酶活性没有十分显著的差别。
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| 关 键 词： | 水稻 过氧化氢酶 逆境 酶活性 |
Isoenzyme of Catalase(CAT) in Rice in Response to Stresses and the Enzymes Activity |
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| Li Yaowen Liu Shenkui Stress Molecular Biology Laboratory,Alkali Soil Natural Environmental Science Center,Northeast Forestry University,Harbin. Isoenzyme of Catalase(CAT) in Rice in Response to Stresses and the Enzymes Activity[J]. Journal of Guangxi Agricultural and Biological Science, 2009, 0(3): 509-514 |
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| Authors: | Li Yaowen Liu Shenkui Stress Molecular Biology Laboratory Alkali Soil Natural Environmental Science Center Northeast Forestry University Harbin |
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| Affiliation: | Li Yaowen Liu Shenkui Stress Molecular Biology Laboratory,Alkali Soil Natural Environmental Science Center,Northeast Forestry University,Harbin,150040 |
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| Abstract: | Catalase is a potent antioxidant enzyme that catalyzes the conversion of H2O2 to oxygen and water.Plants have evolved multiple isoforms of catalase that are differentially regulated in various tissues during development, and by a variety of environmental stresses.In this study, we analyzed the differentiation of the isoenzyme of catalase in rice mRNA expression level by time under abiotic stress(100 mmol/L NaCl, 30 mmol/L NaHCO3, 15 mmol/L Na2CO3, 10% PEG6000, 10% H2O2, low temperature) by RT-PCR.Denaturati... |
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| Keywords: | Rice （Oryza sativa L.） Catalase Stress Enzyme activity |
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