| 碧海万年青和绿大帝蔓绿绒的离体繁殖 |
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| 引用本文： | 张施君,杨承勇,王英强.碧海万年青和绿大帝蔓绿绒的离体繁殖[J].仲恺农业技术学院学报,2001,14(1):32-35. |
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| 作者姓名： | 张施君 杨承勇 王英强 |
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| 作者单位： | 仲恺农业技术学院荫生观赏植物研究中心,广东,广州,510225 |
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| 摘 要： | 以碧海万年青的幼叶和带侧芽的茎段进行离体培养，幼叶在MS＋BAlmg/L＋NAA0.1mg/L的培养基中生长约1周形成愈伤组织，约20d出现丛生芽。碧海万年青茎段侧芽的生长及丛生芽的增殖需要较高浓度的BA。侧芽在添加BA2－4mg/l的MS培养基中约20d可诱导出丛生芽，这苗在1／2MS＋NAA0．2－0．5mg/L培养基中生长约2周可生根，1个月即可出瓶移栽。绿大帝蔓绿绒侧芽在MS＋BA3mg/L的培养基中诱导增殖，生根培养基为1／2MS＋NAA0．2－0．5mg/L，试管苗的移栽成活率可达90％以上。
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| 关 键 词： | 碧海万年青 绿大帝蔓绿绒 组织培养 离体繁殖 观赏植物 |
| 文章编号： | 1006-0774(2001)01-0032-04 |
| 修稿时间： | 2000年10月23 |
Tissue culture for rapid propagation of Dieffenbachia amoena cv.Green Sea and Philodendron erubescens cv.Emerald King |
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| ZHANG Shi jun,YANG Cheng yong,WANG Ying qiang.Tissue culture for rapid propagation of Dieffenbachia amoena cv.Green Sea and Philodendron erubescens cv.Emerald King[J].Journal of Zhongkai Agrotechnical College,2001,14(1):32-35. |
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| Authors: | ZHANG Shi jun YANG Cheng yong WANG Ying qiang |
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| Abstract: | Tissue culture of young leaves and stems of Dieffenbachia amoena cv. Green Sea were studied in present paper. Young leaves formed callus in MS medium culture with 1 mg/L BA and 0.1 mg/L NAA. Cluster buds formed after 20 days. Buds on stem grew and propagated quickly in the medium with higher BA concentration. Seedlings rooted after 2 weeks in rooting medium with extra 0.5 mg/L NAA. Seedlings with roots were cultivated out of cultural bottles after one month. Buds on stem of Philodendron erubescens cv. Emerald King were induced and propagated in MS medium with 3 mg/L BA. 0.5 mg/L NAA was added into rooting MS medium. The survival ratio of tube plantlets was over 90%. |
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| Keywords: | Dieffenbachia amoena cv Green Sea Philodendron erubescens cv Emerald King rapid propagation tissue culture |
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