LRR-RLKs亚家族基因RLK6在拟南芥开花过程中的作用

为研究LRR-RLKs亚家族基因RLK6(AT2G26730)在拟南芥生长发育过程中的作用,本研究构建了植物过表达载体p Super1300-RLK6,获得5个过表达突变体,其中2个独立株系(OE8,OE9)用于表型分析。通过对野生型(WT)、RLK6基因缺失突变体(rlk6-2、rlk6-3和rlk6-6)、过表达株系OE8和OE9生长发育过程的详细观察和分析发现,RLK6基因缺失突变体在整个生长发育过程中的表型与WT差异不明显,而过表达株系OE8和OE9比WT开花早。此外,利用实时定量PCR(qRT-PCR)检测各基因型植株中开花相关基因的表达情况,结果表明,相对于WT,OE8和OE9中的开花促进基因CO、SOC1和LFY的表达量显著升高,而开花抑制基因FLC的表达量显著下降。研究结果说明RLK6过表达促进了拟南芥的开花,这为后续深入研究RLK6的作用机制奠定了基础。

Study of RLK6, One Member of Leucine-rich Repeat Receptor-like Kinases (LRR-RLKs) Subfamily Genes,on Process of Flowering in Arabidopsis

To investigate the functions of LRR-RLKs gene RLK6 in Arabidopsis,a pSuper1300-RLK6 expression vector was constructed and five RLK6 overexpression transgenic lines were obtained.The two lines,OE8 and OE9,were selected for further analysis.The homozygous RLK6 mutants (rlk6-2,rlk6-3 and rlk6-6),OE8,OE9 and WT were characterized for phenotype analysis,and the result showed that RLK6 mutants appeared to show similar phenotype with wild-type (WT) during growth and development.But,OE lines displayed obviously early flowering than WT.Four key genes which relates to flowering were examined in all genotypes by using qRT-PCR.In OE lines,increased expression of floral activator genes (CO,SOC1 and LFY) with the decreased level of floral repressor gene (FLC) was observed,which was consistent with it phenotypes,when compared to WT.These results suggest that RLK6 play a key role in promoting flowering in Arabidopsis,and these results will lay the foundation for understanding the mechanisms of RLK6.