| 小鼠腔前卵泡卵母细胞的采集、体外发育和体外成熟 |
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| 引用本文： | 芮荣,张涌.小鼠腔前卵泡卵母细胞的采集、体外发育和体外成熟[J].四川农业大学学报,1998,16(3):349-351. |
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| 作者姓名： | 芮荣 张涌 |
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| 作者单位： | 1. 四川农业大学动物科技学院/雅安市,625014
2. 西北农业大学动物医学系/陕西杨陵,712100 |
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| 基金项目： | 国家自然科学基金,农业部生物技术重点项目 |
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| 摘 要： | 采用机械法分离成年小鼠和１３日龄小鼠腔前卵泡，两组中每只小鼠平均回收率分别为４９２９±１０８１和７６８３±９１５个；腔前卵泡卵母细胞培养１０天后，两组恢复成熟分裂卵的比例分别为１９３％和２０９％。试验结果表明，小鼠腔前卵泡卵母细胞在培养后第５天开始发生ＧＶＢＤ，此后，ＧＶＢＤ和抛出ｐｂＩ的卵母细胞数量逐日增加。
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| 关 键 词： | 腔前卵泡 采集 体外成熟 小鼠 |
COLLECTION, IVD AND IVM OF PREANTRAL FOLLICULAR OOCYTES IN MOUSE |
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| Rui Rong,Zhang Yong.COLLECTION, IVD AND IVM OF PREANTRAL FOLLICULAR OOCYTES IN MOUSE[J].Journal of Sichuan Agricultural University,1998,16(3):349-351. |
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| Authors: | Rui Rong Zhang Yong |
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| Institution: | Rui Rong 1 Zhang Yong 2 |
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| Abstract: | Preantral follicles were isolated from mouse ovaries by the mechanical method. In adult and 13-day-old mouse, the average recovery rate of preantral follicles was 49·29±10 81 and 76 83±9 15 per mouse, respectively. After the oocytes from preantral follicles were cultured for 10 days, the percentage of oocytes resuming meiosis in these two groups was 19 3% and 20 9%, respectively. It shows that GVBD began to occur in mouse oocytes from preantral follicles after culture on the 5th day in vitro. Thereafter, the number of oocytes generated GVBD and pbI increased day by day. |
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| Keywords: | PREANTRAL FOLLICLE COLLECTION IN VITRO MATURATION MOUSE |
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