萨×哈杂交母羊GHRH基因与生长性状关联分析

为探讨GHRH基因作为绵羊生长性状候选基因的可能性,寻找与绵羊生长性状相关的分子标记,对萨福克羊与哈萨克羊杂交成年母羊GHRH基因rs400079090和rs419322328位点的多态性及其与生长性状的关联性进行了研究。结果表明：rs400079090位点检测到3个基因型,rs419322328位点TT基因型缺失;rs419322328位点GG基因型个体胸宽显著大于GT基因型个体（P〈0.05）,体斜长极显著小于GT基因型个体（P〈0.01）。说明GHRH基因2个SNP位点多态性对萨福克羊与哈萨克羊杂交成年母羊生长性状有一定的影响,可以作为进行绵羊生长性状分子标记辅助选择的候选基因。

Correlation Analysis between GHRH Gene and Growth Traits in Suffolk and Kazak Crossbred Ewes

This study was aimed to investigate the relationship between GHRH gene and sheep growth traits and to search the potential molecular marker associated with the growth traits of sheep. The polymorphisms of rs400079090 and rs419322328 loci of G H RH gene in Suffolk and Kazak crossbred adult ewes were determined. Furthermore, the association of their polymorphisms with the growth traits of the ewes was assessed. The results showed that 3 genotypes were identified at rs400079090 locus and TT genotype was absent at rs419322328 locus; the individuals of GG genotype at rs419322328 locus had significantly higher chest width（P〈0.05） and extremely significantly lower body length（P〈0.01 ）compared to those of GT genotype. In conclusion, effects of two SNPs of GHRH gene on the growth traits of Suffolk and Kazak crossbred adult ewes were identified in this study, and the GH RH gene could serve as a candidate gene for marker-assisted selection of sheep growth traits.