甘薯抗薯瘟病的苯丙烷类代谢研究

甘薯瘟病是甘薯的重要病害, 严重影响甘薯的品质和产量.本试验在甘薯苗期, 通过剪叶法接菌研究甘薯抗感病品种苯丙烷类代谢的变化规律.结果表明: 甘薯抗感病品种接菌后PAL、POD活性均有所提高, 且提高速度与品种抗性呈正相关; 抗病品种接菌后木质素和绿原酸含量显著增加, 感病品种接菌后绿原酸、总酚和类黄酮含量波动较大, 木质素含量降低.相关性分析表明, 接菌后木质素和绿原酸的积累与甘薯品种抗病性密切相关.接菌后PAL、POD活性迅速提高并积累木质素和绿原酸等抗病物质是甘薯抗薯瘟病的苯丙烷类代谢基础, 在抗病品种鉴定方面有一定的参考价值.

Phenylaprapanoid metabolism of sweet potato against Pseudomonas solanacearum

Pseudomonas solanacearum is a major diseases in sweet potato which leads to substantive yield loss and quality degradation. Changes in phenylaprapanoid metabolism were studied in resistant and susceptible cultivars of sweet potato seedlings after inoculation by leaf-cut. The results indicate that PAL and POD activities in resistant and susceptible cultivars increase after inoculation. However, the rates of increase in PAL and POD activities are directly related with cultivar resistance to pathogens. Lignin and chlorogenic acid contents increase significantly after inoculation of resistant cultivars. Though chlorogenic acid, total phenolics and flavonoids contents largely fluctuate after inoculation, and lignin content decreases in susceptible cultivars. The relative analysis shows that the accumulation of lignin and chlorogenic acid has close correlation with cultivar resistance. Rapidly increase in PAL and POD activity and in the accumulation of lignin and chlorogenic acid after inoculation is the basis for phenylaprapanoid metabolism against P. solanacearum in sweet potato. This can find application in referencing resistant cultivar identification.