| AB-8大孔树脂纯化鸡骨草总皂苷的工艺研究 |
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| 引用本文： | 廖春燕,黄瑶,杨晓.AB-8大孔树脂纯化鸡骨草总皂苷的工艺研究[J].河南农业科学,2013,42(3). |
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| 作者姓名： | 廖春燕 黄瑶 杨晓 |
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| 作者单位： | 广西工学院生物与化学工程系,广西柳州,545006 |
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| 基金项目： | 广西科技大学(筹)科学基金资助项目 |
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| 摘 要： | 为了提高鸡骨草总皂苷的纯化效率,对AB-8大孔树脂纯化鸡骨草总皂苷的工艺条件及技术参数进行研究。以总皂苷吸附率、比吸附量和保留率为指标,考察AB-8大孔树脂对鸡骨草总皂苷的吸附及解吸性能。结果表明,鸡骨草皂苷提取液的上样质量浓度为1.00g/L,pH值为6.0,上样速度为2.0BV/h,上样量为2.0BV;吸附平衡后用3.5BV pH值为7.0的50%乙醇溶液洗脱,洗脱速度为4.0BV/h,总皂苷保留率为82.8%,纯化后纯度达到75.18%。
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| 关 键 词： | 鸡骨草 总皂苷 AB-8大孔树脂 纯化 |
Purification Process of Total Saponins in Abrus cantoniensis Using AB-8 Macroporous Resin |
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| LIAO Chun-yan
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HUANG Yao
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YANG Xiao.Purification Process of Total Saponins in Abrus cantoniensis Using AB-8 Macroporous Resin[J].Journal of Henan Agricultural Sciences,2013,42(3). |
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| Authors: | LIAO Chun-yan HUANG Yao YANG Xiao |
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| Abstract: | In order to improve the purification efficiency of total saponins in Abrus cantoniensis,the conditions and parameters of purification process of total saponins using AB-8 macroporous adsorption resin were studied.The conditions of absorption and desorption were investigated with the indexes:absorption ratio and reserved rate of total saponins.The results showed that the sample loading solution was 1.00 g/L of 2.0 BV with pH value of 6.0 at the sample loading flow rate of 2.0 BV/h.The elution solvent was 3.5 BV of 50% ethanol solution with pH value of 7.0 at flow rate of 4.0 BV/h.The total saponins reserved rate was 82.8%,with the purity of 75.18%. |
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| Keywords: | Abrus cantoniensis total saponins AB-8 macroprous resin purification |
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