鸭IL-18基因克隆与序列分析

[目的]为深入研究鸭IL-18的基因表达及其生物学活性和分子佐剂的应用奠定基础。[方法]依据GenBank中香港鸭IL-18基因的序列设计1对特异引物,以广东水鸭脾淋巴细胞总RNA为模板进行RT-PCR扩增,克隆广东水鸭IL-18基因并进行序列分析。[结果]测序结果表明,广东水鸭的IL-18基因开放阅读框为603 bp,编码201个氨基酸,分子量为23.2 kDa。序列分析表明,所获得的广东水鸭IL-18基因与GenBank中香港鸭的核苷酸同源性为98.2%,氨基酸同源性为100%。它与鸡和火鸡的核苷酸同源性分别为86.9%和87.1%,而氨基酸同源性分别为96.5%和97.0%。[结论]进化树分析结果表明,鸭IL-18基因与鸡和火鸡进化关系较近。

Cloning and Sequence Analysis of Duck IL-18 Gene

[Objective] The research aimed to lay the foundation for deeply studying the expression of duck IL-18 gene and its biological activity and molecular adjuvant application.[Method] A pair of primers was designed according to the sequences of IL-18 gene in the duck of Hongkong on GenBank website.Total RNA from spleen lymphocyte in Guangdong teal was taken as template to make RT-PCR amplification.IL-18 gene in Guangdong teal was cloned and sequenced.[Result] The sequencing results showed that the open reading frame of IL-18 gene in Guangdong teal was 603 bp,coding 201 amino acids,with the molecular weight of 23.2 kDa.The sequence analysis showed the nucleotide homology of IL-18 gene between Guangdong teal and Hongkong duck on GenBank website was 98.2% and the amino acid homology was 100%.Its nucleotide homology with chicken and turkey were 86.9% and 87.1% resp.,the amino acid homology were 96.5% and 97.0% resp.[Conclusion] The results of phylogenetic analysis showed that duck IL-18 gene had closer evolutionary relationships with that of chicken and turkey.