| 采用ISSR-PCR分子标记法鉴别黑果枸杞与雄性不育枸杞 |
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| 引用本文： | 思彬彬,张靠稳,刘国迪. 采用ISSR-PCR分子标记法鉴别黑果枸杞与雄性不育枸杞[J]. 安徽农业科学, 2012, 40(33): 16094-16095 |
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| 作者姓名： | 思彬彬 张靠稳 刘国迪 |
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| 作者单位： | 北方民族大学生物科学与工程学院,宁夏银川,750021;北方民族大学生物科学与工程学院,宁夏银川,750021;北方民族大学生物科学与工程学院,宁夏银川,750021 |
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| 摘 要： | [目的]探索用ISSR分子标记方法在核酸分子水平上鉴别黑果枸杞与雄性不育枸杞。[方法]从100条ISSR引物中筛选合适的引物对黑果枸杞与雄性不育枸杞样品进行PCR扩增及电泳分析,寻找特征位点。[结果]有4条ISSR引物扩增出较为明显的多态性特征条带,可单独应用于黑果枸杞与雄性不育枸杞的鉴别。[结论]ISSR作为一种简便、可靠的分子标记方法,可用于黑果枸杞与雄性不育枸杞的鉴别。
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| 关 键 词： | 枸杞 ISSR 鉴别 |
Identification of Lycium ruthenicum Murr and Male Sterility of Lycium barbarum L. by Inter-Simple Sequence Repeat Markers |
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| Affiliation: | SI Bin-bin et al(College of Biological Science and Engineering,Beifang University for Nationalities,Yinchuan,Ningxia 750021) |
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| Abstract: | [Objective] To identify Lycium ruthenicum and male sterility of Lycium barbarum by inter-simple sequence repeat markers.[Method] Primers suitable for routine analysis were screened from 100 inter-simple sequence repeat primers,then,they were used in PCR and separated of 2 samples of L.ruthenicum and male sterility of L.barbarum L.[Results] Four of the 100 primers amplified polymorphic bands and suitable for the identification of L.ruthenicum and male sterility of L.barbarum.[Conclusion] Inter-simple sequence repeat markers provide a quick,reliable molecular marker for identification of L.ruthenicum and male sterility of L.barbarum. |
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| Keywords: | Lycium barbarum L. ISSR Identification |
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