不同发育类型的麦冬块根中多糖含量变化规律的比较

目的]研究国产优质麦冬块根不同发育阶段的麦冬多糖含量的相关性规律,确定其品质特征,为麦冬GAP质量控制及资源综合开发利用提供科学依据。方法]在杭麦冬(3年生)和川麦冬(1年生)主产区采集植株块根样品,按照鲜块根等级划分标准分级,微波杀酶-50℃恒温烘干,并采用苯酚-硫酸比色法分别测定各等级麦冬块根的多糖含量;运用SPSS17.0版统计软件分析不同发育阶段麦冬块根平均生物量与麦冬多糖含量的相关性规律。结果]不同发育阶段的麦冬块根的多糖平均含量分别为24.36%～41.09%(杭麦冬)和21.54%～40.29%(川麦冬);随着麦冬植株块根发育阶段进程增加,麦冬块根的光合产物积累量与麦冬多糖含量呈极显著的正相关增长(P<0.001),其回归方程分别为:杭麦冬y1=7.496ln(x)+36.45(r2=0.993),川麦冬y2=12.68x+19.22(r2=0.997)。结论]麦冬块根阶段发育的光合产物积累量与麦冬次生代谢产物多糖的含量密切相关,国产麦冬块根的传统等级规格的划分是科学的。

A Preliminary Study of the Variation of the Polysaccharide Content of Domestic High Quality Ophiopogon japonicus (Thunb) Ker-Gawl.Tubers at Different Development Stages

Objective] To study the variation of the polysaccharide content of domestic high quality Ophiopogon japonicus tubers at different developmental stages,to determine the quality characteristics and to provide a scientific basis for the GAP quality control and integrated resource development and utilization of O.japonicus.Method] Plant tubers collected from Hangzhou O.japonicus(3 years old) and Sichuan Ophiopogon japonicus(1 year old) were graded in accordance with the fresh tuber grading standards.Through microwave kill enzyme-50 ℃ and constant temperature drying,the polysaccharide content of O.japonicus tubers was determined by phenol-sulfuric acid colorimetric method.The law of correlation between the average biomass and the polysaccharide content of tubers at different developmental stages was analyzed using SPSS17.0 statistical software.Result] The polysaccharide content of Hangzhou O.japonicus and Sichuan O.japonicus tubers at different developmental stages ranged from 24.36% to 41.09% and from 21.54% to 40.29%,respectively.With the increase of developmental stage,the accumulation of photosynthetic products was significantly positively related to the polysaccharide content of O.japonicus tubers(P<0.001***).The regression equation was written as y1=7.496ln(x) + 36.45(R2=0.993***) for Hangzhou O.japonicus,and y2=12.68x+19.22(R2= 0.997***) for Sichuan O.japonicus.Conclusion] the accumulation of photosynthetic products in O.japonicus tubers at different developmental stages is closely related to the content of secondary metabolites polysaccharide.It is scientific to divide the domestic high quality O.japonicus tubers by the traditional grade specifications.