Estrogen formation by the isolated perfused rhesus monkey brain

Perfusion of two isolated brains from immature male rhesus monkeys with (3)H]androstenedione resulted in the identification of free and conjugated (3)H]estrone and free (3)H]estradiol from the perfusates. In the dissected cerebral tissues, estrogens were recovered only from the hypothalamus and limbic system. The production of estrogens from androstenedione during the 40-minute perfusions in these two experiments totaled 1.58 and 2.83 nanograms.