| CHO表达生长激素释放因子的生物活性测定 |
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| 引用本文： | 任晓慧,刘松财,张永亮,欧阳红生,赵建军,张玉静.CHO表达生长激素释放因子的生物活性测定[J].吉林农业大学学报,2003,25(6):672-673. |
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| 作者姓名： | 任晓慧 刘松财 张永亮 欧阳红生 赵建军 张玉静 |
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| 作者单位： | 1. 河北农业大学水产学院,河北,秦皇岛,066003
2. 解放军军需大学基础部,吉林,长春,130062 |
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| 基金项目： | 国家自然科学基金(39500105) |
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| 摘 要： | 在对生长激素释放因子(GRF)基因改造和化学合成，并构建了其真核表达载体pCDNA3-GRF(1-32)的基础上．用Lipofectin将上述载体转染CHO细胞进行瞬时表达，采用体外单层垂体细胞培养的方法对表达的GRF(1-32)进行了体外生物活性测定，即先将垂体用酶进行消化，再将消化细胞培养，形成单层细胞，利用其能合成与释放生长激素的能力来检测GRF的活性。结果表明：表达产物可刺激生长激素释放，并且比对照组提高3．8倍，
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| 关 键 词： | 生长激素释放因子 垂体单层细胞培养 CHO细胞 瞬时表达 生物活性 体外测定 生长激素 |
| 文章编号： | 1000-5684(2003)06-0672-02 |
| 修稿时间： | 2003年5月8日 |
Determination of the Activity of Growth Hormone Releasing Factor Expressed in CHO Cells |
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| REN Xiao-hui,LIU Song-cai,ZHANG Yong-liang,OYANG Hong-sheng,ZHAO Jian-jun,et al..Determination of the Activity of Growth Hormone Releasing Factor Expressed in CHO Cells[J].Journal of Jilin Agricultural University,2003,25(6):672-673. |
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| Authors: | REN Xiao-hui LIU Song-cai ZHANG Yong-liang OYANG Hong-sheng ZHAO Jian-jun |
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| Institution: | REN Xiao-hui~1,LIU Song-cai~2,ZHANG Yong-liang~2,OYANG Hong-sheng~2,ZHAO Jian-jun~2,et al. |
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| Abstract: | Chemical synthesis of the GRF(1-32) gene had been finished in our former work. GRF(1032) gene, synthesized in this laboratory previously, was inserted into a plasmid , pcDNA3, to construct an expression vector in CHO cells. The expression vector was purified with a High Pure Plasmid Extraction Kit and then trasfected into prepared CHO cells in 60% confluent. Dispersed rat pituitary was used to determine the activity of expressed GRF(1-32) . The results showed that the expressed products stimulated Growth Hormone(GH) secretion in pituitary cells at high levels, being 3.8 times higher than the control group. |
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| Keywords: | GRF dispersed pituitary cell culture GH determination of activity |
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