甜橙油抗氧化活性研究

目的]研究甜橙油的抗氧化活性。方法]分别采用DPPH自由基清除法和普鲁士蓝法测定甜橙油的抗氧化活性。结果]甜橙油的质量浓度小于192.5 mg/ml时,甜橙油对DPPH自由基的清除率呈剂量效应增加;当其质量浓度超过192.5 mg/ml时,甜橙油对DP-PH自由基的清除率基本维持不变。在27.5～192.5 mg/ml的浓度范围内,DPPH自由基清除率与甜橙油浓度呈现良好的量效关系：y=0.3192x＋28.9（R2=0.987 1）。DPPH自由基清除试验中,以518 nm为检测波长,反应平稳时间为60 minI,C50值为（66.10±0.39）mg/ml。在27.5～137.5 mg/ml范围内甜橙油的还原能力呈线性增高趋势,其线性拟合方程为y=0.004 1x＋0.121 1（R2=0.989 9）,证实甜橙油具有较强的还原性,是比较好的电子供应者。结论]甜橙油具有较强的还原能力,其还原力与质量浓度在27.5～137.5 mg/ml范围内存在极显著的量效关系。

Study on the Antioxidant Activity of Sweet Orange Oil

Objective] The purpose was to study the antioxidant activity of sweet orange oil.Method] The antioxidant activity of sweet orange oil was determined by DPPH free radical elimination method and Prussian blue method resp.Result] When the mass concn.of sweet orange oil was less than 192.5 mg/ml and the sweet orange oil showed dose effect on increasing the eliminated rate of DPPH free radical.When its mass concn.exceeded 192.5 mg/ml,the eliminated rate of DPPH free radical was invariable basically.When its concn.was in 27.5-192.5 mg/ml,the eliminated rate of DPPH free radical showed good dose-effect relationship with the concn.of sweet orange oil and their linear equation was y=0.319 2x+28.9,(R2=0.987 1).In the experiment on eliminating DPPH free radical,the detection wavelength was 518 nm,the stabilizing time of reaction was 60 min and IC50 was(66.10±0.39) mg/ml.In the concn.range of 27.5-137.5 mg/ml,the reducing power of sweet orange oil was increased linearly along with the increase of its concn.and its line fitting equation was y=0.004 1x+0.121 1,(R2=0.989 9).So,it was validated that the sweet orange oil possessed higher reducing power and was better electron supplier.Conclusion] The sweet orange oil possessed higher reducing power and its reducing power showed extremely significant dose-effect relationship with its mass concn.in the range of 27.5-137.5 mg/ml.