| 鸡基因组DNA不同提取方法的比较研究 |
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| 引用本文： | 马新红,亢娟娟,康相涛,韩瑞丽,孙桂荣,潘军,黄煌,刘凯,张立恒.鸡基因组DNA不同提取方法的比较研究[J].江西农业大学学报,2010,32(1). |
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| 作者姓名： | 马新红 亢娟娟 康相涛 韩瑞丽 孙桂荣 潘军 黄煌 刘凯 张立恒 |
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| 作者单位： | 河南农业大学,牧医工程学院,河南,郑州,450002 |
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| 基金项目： | 国家科技支撑计划项目(2006BDA01A09);;公益性行业(农业)科研专项(nyhyzx07-039);;河南省杰出人才创新基金项目(074200510009) |
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| 摘 要： | 实验用优化煮沸法提取鸡血液基因组DNA,经核酸蛋白定量仪检测,DNA的OD260/OD280达1.77±0.06,证明所提DNA质量较好,用于PCR扩增,可以得到满意的扩增效果。与酚-氯仿法相比,优化煮沸法避免了用氯仿、酚等剧毒试剂,且节约了时间和成本,是一种较好的提取基因组DNA的方法。
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| 关 键 词： | 鸡 基因组DNA PCR扩增 提取方法 |
A Comparative Study on Different Methods for Extraction of Gallus Genomic |
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| MA Xin-hong,KANG Juan-juan,KANG Xiang-tao,HAN Rui-li,SUN Gui-rong,PAN Jun,HUANG Huang,LIU Kai,ZHANG Li-heng.A Comparative Study on Different Methods for Extraction of Gallus Genomic[J].Acta Agriculturae Universitis Jiangxiensis,2010,32(1). |
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| Authors: | MA Xin-hong KANG Juan-juan KANG Xiang-tao HAN Rui-li SUN Gui-rong PAN Jun HUANG Huang LIU Kai ZHANG Li-heng |
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| Institution: | College of Animal Science And Veterinary Medicine/a>;HAU/a>;Zhengzhou 450002/a>;China |
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| Abstract: | The optimized cooking extraction method was used in this study to extract gallus genomic DNA.OD_(260)/OD_(280) was up to 1.77±0.06 by the SmartspecTMplus test.The result showed that the quality of DNA extracted by the optimized cooking extraction method was very good,clear and bright fragments were detected through PCR amplification.Compared with the Phenol-chloroform extraction method,The optimized cooking extraction method is free from chloroform,phenol and other toxic reagents,and saves time and costs,hence a good method for extracting genomic DNA. |
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| Keywords: | gallus genomic DNA PCR amplification extraction method |
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