| 棉花盐胁迫应答基因GhCPK5的克隆及序列分析 |
| |
| 引用本文： | 甄军波,张曦,王玉美,翟志席,李召虎,华金平.棉花盐胁迫应答基因GhCPK5的克隆及序列分析[J].中国农业大学学报,2011,16(3):8-14. |
| |
| 作者姓名： | 甄军波 张曦 王玉美 翟志席 李召虎 华金平 |
| |
| 作者单位： | 1. 中国农业大学农学与生物技术学院/农业部作物基因组学与遗传改良重点开放实验室/作物改良北京市重点实验室,北京,100193
2. 湖北省农业科学院经济作物研究所,武汉,430064 |
| |
| 基金项目： | 国家“863”计划项目(2006AA10A108); 教育部新世纪优秀人才支持计划项目(NCET-06-0106) |
| |
| 摘 要： | 本研究克隆了1个陆地棉新的钙依赖蛋白激酶基因GhCPK5(Genbank登录号为:HM002634)。GhCPK5基因组DNA序列全长3 031 bp,包含一个1 707 bp的开放阅读框,具有7个外显子和6个内含子。GhCPK5可能定位于细胞核内,有568个氨基酸残基,分子量为63 ku。氨基酸序列分析表明,陆地棉GhCPK5与杨树PtCPK5同源性达到89%,同样包含完整的激酶结构域和4个EF-hand结构域。RT-PCR分析表明,该基因在根、茎、叶等组织中表达;在盐胁迫处理下,GhCPK5在各组织的表达量明显上升。
|
| 关 键 词： | 棉花 GhCPK5 盐胁迫应答 RACE 序列分析 |
| 收稿时间： | 2011/1/25 0:00:00 |
Isolation and sequence analysis of salt responsive geneGhCPK5 from Gossypium hirsutum L. |
| |
| ZHEN Jun-bo,ZHANG Xi,WANG Yu-mei,ZHAI Zhi-xi,LI Zhao-hu and HUA Jin-ping.Isolation and sequence analysis of salt responsive geneGhCPK5 from Gossypium hirsutum L.[J].Journal of China Agricultural University,2011,16(3):8-14. |
| |
| Authors: | ZHEN Jun-bo ZHANG Xi WANG Yu-mei ZHAI Zhi-xi LI Zhao-hu and HUA Jin-ping |
| |
| Institution: | ZHEN Jun-bo1,ZHANG Xi1,WANG Yu-mei2,ZHAI Zhi-xi1,LI Zhao-hu1,HUA Jin-ping1(1.College of Agronomy and Biotechnology/Key Laboratory of Crop Genomics and Genetic Improvement of Ministry ofAgriculture/Beijing Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100193,China,2.Institute of Cash Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China) |
| |
| Abstract: | In this study,a salt responsive gene GhCPK5was firstly obtained from upland cotton using RT-PCR and RACE.GhCPK5located probably in cell nucleus and the genomic sequence indicated that GhCPK5had 7 exons and 6 introns.GhCPK5contained an open reading frame(ORF) of 1 707 bp,which encoded a protein with 568 amino acids,with a molecular weight of 63 ku.GhCPK5 contained one protein kinase domain and four conserved EF-hand domains.The amino acid sequence of GhCPK5 shared 89% identify with Populus trichocarpa homolo... |
| |
| Keywords: | cotton GhCPK5 salt stress response RACE sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国农业大学学报》浏览原始摘要信息 |
| 点击此处可从《中国农业大学学报》下载免费的PDF全文 |
|
