| 小麦幼苗对镉和1,2,4-三氯苯污染的响应 |
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| 引用本文： | 丁艳,葛才林,王泽港,杜庆才.小麦幼苗对镉和1,2,4-三氯苯污染的响应[J].中国农业大学学报,2011,16(3):48-52. |
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| 作者姓名： | 丁艳 葛才林 王泽港 杜庆才 |
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| 作者单位： | 1. 蚌埠学院应用化学与环境工程系,安徽,蚌埠,233000
2. 扬州大学生物科学与技术学院,江苏,扬州,225009 |
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| 基金项目： | 蚌埠学院自然科学重点项目(2010ZR02zd); 江苏省作物栽培生理重点实验室开放课题(K09009) |
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| 摘 要： | 应用双向凝胶电泳技术,结合含水率及膜脂过氧化物测定等方法,研究镉(Cd)和1,2,4-三氯苯(TCB)污染对扬麦13幼苗生长的影响。结果表明:Cd和TCB污染造成扬麦13幼苗含水率降低,导致膜脂过氧化。Cd和TCB污染对小麦叶片中的蛋白质组分也有较大影响:与对照相比,污染处理后的叶片蛋白组分中检测出12个TCB胁迫诱导蛋白和10个Cd胁迫诱导蛋白,其中有些蛋白是TCB特异性诱导蛋白,也有一些蛋白为Cd2+胁迫特异性诱导蛋白。这些诱导蛋白的产生可能是扬麦13对Cd或TCB污染的一种适应性响应。
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| 关 键 词： | 小麦 镉 1 2 4-三氯苯 蛋白质组 污染 |
| 收稿时间： | 2010/9/26 0:00:00 |
Response of Cd and 1,2,4-Trichlorobenzene pollutantson growth of wheat seedlings |
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| DING Yan,GE Cai-lin,WANG Ze-gang and DU Qing-cai.Response of Cd and 1,2,4-Trichlorobenzene pollutantson growth of wheat seedlings[J].Journal of China Agricultural University,2011,16(3):48-52. |
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| Authors: | DING Yan GE Cai-lin WANG Ze-gang and DU Qing-cai |
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| Institution: | DING Yan1,GE Cai-lin2,WANG Ze-gang2,DU Qing-cai1(1.Department of Chemistry and Environmental Engineering,Bengbu College,Bengbu 233000,China,2.College of Biological Sciences and Biotechnology,Yangzhou University,Yangzhou 225009,China) |
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| Abstract: | The effects of wheat seedlings to Cd and 1,2,4-trichlorobenzene(TCB) pollutants were investigated by methods of Two-dimensional Electrophoresis,combined with water content,and lipid peroxide determination.The results showed that Cd and TCB pollutants inhibited the growth of leaves and roots which ledding to water deficit and causing lipid peroxidation in wheat s seedlings.Moreover,Cd and TCB stress had significant effect on global proteome in wheat leaves.Comparing with the contrast,we found that there were... |
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| Keywords: | wheat Cd2 TCB proteome pollutant |
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