鸭白细胞抗菌肽对小鼠免疫功能的影响

从樱桃谷鸭血液中提取出白细胞并制备粗提抗菌肽,分别以低、中、高3种剂量(质量浓度分别为1.5 g/L、7.5 g/L、15 g/L)灌胃清洁级昆明系小鼠,0.2 mL/(d·只),同时设0.0l%醋酸溶液对照组和蛋白对照组(10 g/L牛血清白蛋白).连续灌胃14 d后,测定小鼠的免疫器官指数、E玫瑰花环形成率、碳粒廓...

Effects of Antimicrobial Peptides from Duck Leukocytes on Immunity of Mice

Crude extractions of antimicrobial peptides from Yingtaogu duck blood was administrated in Kunming strain mice for two weeks through gavage at a daily dose of 0.2mL with the concentrations of 1.5g/L,7.5g/L or 15g/L,respectively.For negative controls,0.2mL of 0.01% acetic acid or 10g/L BSA was separately used.The serum phagocytic rate,phagocytic index,and weight of the immune organs(spleen and thymus) were measured,whilst the total number and subgroups of tlymphocytes were counted through flow cytometry(FCM).The results showed that the extract improved the E-rosette ratio and the total number of CD3+ T cells.Compared to other groups,the extract used at a dosage of 15mg/L significantly improved the immune organ weight index(P<0.05),serum phagocytic index(P<0.01),and the percentages of both CD4+ and CD8+ T cells(P<0.01).This indicates that the immunity of mice was significantly improved by antimicrobial peptides extracted from duck leukocytes.