| 茶树ACC氧化酶基因全长cDNA的克隆与表达分析 |
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| 引用本文： | 张亚丽,乔小燕,陈亮. 茶树ACC氧化酶基因全长cDNA的克隆与表达分析[J]. 茶叶科学, 2008, 28(6) |
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| 作者姓名： | 张亚丽 乔小燕 陈亮 |
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| 作者单位： | 中国农业科学院茶叶研究所茶树资源与改良研究中心/国家茶树改良中心,浙江,杭州310008 |
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| 基金项目： | 国家863计划，浙江省"钱江人才"计划项目(2006R10042)内容之一 |
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| 摘 要： | ACC(1-氨基环丙烷-1-羧酸)氧化酶是植物乙烯合成过程中的关键酶之一,对乙烯的合成具有重要的调控作用。在茶树新梢cDNA文库测序所获得ESTs基础上,利用RT-PCR技术,克隆得到编码ACC氧化酶基因的全长序列,GenBank登录号为DQ904328。该基因长1232bp,编码320个氨基酸残基,预测分子量为36.2kD,等电点5.41。多序列联配表明茶树ACC氧化酶具有高度保守区域,基于邻接法的进化树显示与柿树ACC氧化酶的亲缘关系最近。对经高、低温后的不同品种进行RT-PCR分析,结果发现ACC氧化酶基因的表达量与品种的抗逆性有一定的相关性。
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| 关 键 词： | 茶树 乙烯 ACC氧化酶 基因克隆 表达分析 |
Molecular Cloning and Expression Analysis on ACC Oxidase Gene Full-length cDNA from Tea Plant |
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| ZHANG Ya-li,QIAO Xiao-yan,CHEN Liang. Molecular Cloning and Expression Analysis on ACC Oxidase Gene Full-length cDNA from Tea Plant[J]. Journal of Tea Science, 2008, 28(6) |
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| Authors: | ZHANG Ya-li QIAO Xiao-yan CHEN Liang |
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| Affiliation: | ZHANG Ya-li,QIAO Xiao-yan,CHEN Liang (Research Center for Tea Germplasm , Improvement,Tea Research Institute Chinese Academy of Agricultural Sciences,National Center for Tea Improvement,Hangzhou 310008,China) |
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| Abstract: | 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase is an important enzyme and plays a critical regulatory role in the biosynthesis of ethylene. Based on previous ESTs sequencing results of the tender shoots cDNA library and RT-PCR technology, a full-length cDNA sequence coding ACC oxidase (ACO) of tea plant was cloned. The GenBank accession is DQ904328 in the NCBI. The ACO gene had 1 232 bp in length, encoding 320 amino acid residues with the putative molecular weight of 36.2 KD and the pI 5.41. The protei... |
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| Keywords: | tea plant (Camellia sinensis) ethylene ACC oxidase gene cloning expression analysis |
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