荔枝品种亲缘关系的AFLP分析

 应用AFLP技术，对39个荔枝品种进行了遗传多样性分析及分类研究。筛选出适于荔枝AFLP分析的最佳引物组合3对，分别为EcoRI AAC+MseI CTG，EcoPd AGC+MseI CAT，EcoRI ACC+Msel CAT。在分子水平上，荔枝品种的遗传多样性并非形态学性状所体现的那样丰富。AFLP分析将39个荔枝品种分成了6组，与传统的以果皮龟裂片隆起类型为分类标准的分类没有一致性。初步建立起荔枝主要栽培品种的分子标记标准图谱。应用AFLP技术对来自两个不同地方的两个荔枝品种(桂味、妃子笑)进行了鉴别。

Studies on Genetic Relationship among Litchi Varieties by Using AFLP

Identification and classification of 39 litchi varieties were studied based on AFLP analysis.Three pair of primers( Eco RI AAC+ Mse I CTG, Eco RI AGC+ Mse I CAT, Eco RI ACC+ Mse I CAT)were screened from 64 pare of primers.The analysis of genetic diversity among litchi varieties indicated on morphological level less abundant than molecular level.The cluster analysis results showed that the 39 cultivars could be divided into 6 groups.It is different from the traditional classification system based on peel morphology including peel smoothness,scale size,scale arrangement.The results showed peel morphology can not be used for litchi varieties classification as the only criterion.The molecular marker standard map of the main litchi varieties was established.Two varieties( Litchi chinensis 'Guiwei'and Litchi chinensis 'Feizixiao')come from different area were identified.