| 苹果新品种自交不亲和基因型的分子生物学鉴定 |
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| 引用本文： | 张雪梅,李保国,崔惠英,齐国辉.苹果新品种自交不亲和基因型的分子生物学鉴定[J].园艺学报,2007,34(3):747-750. |
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| 作者姓名： | 张雪梅 李保国 崔惠英 齐国辉 |
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| 作者单位： | ( 河北农业大学西校区林学院, 河北保定071000; 河北省林业技术推广总站, 石家庄050081) |
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| 基金项目： | 河北农业大学校科研和教改项目 |
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| 摘 要： | 根据保守氨基酸序列FTQQYQ 和anti-1/M IWPNV 设计苹果自交不亲和基因引物P1 : 5′2 TT2TACGCAGCAATATCAG23′; P2 : 5′2 ACGTTCGGCCAAATA /CATT23′, 利用PCR - RFLP分析方法, 以河北省新引进的苹果品种美国8号、昂林、松本锦、华冠、南方脆幼叶为试验材料, 鉴定其自交不亲和基因型分别为S9S27、S1S9、S9S 9、S2S9、S2S9。
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| 关 键 词： | 苹果 自交不亲和基因型 分离 鉴定 |
| 文章编号： | 0513-353X（2007）03-0747-04 |
| 修稿时间： | 2006-10-242007-05-08 |
Molecular Biology Identification of Self-incompatibility Genotypes of New Apple Cultivars |
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| ZHANG Xue-mei,LI Bao-guo,CUI Hui-ying,QI Guo-hui.Molecular Biology Identification of Self-incompatibility Genotypes of New Apple Cultivars[J].Acta Horticulturae Sinica,2007,34(3):747-750. |
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| Authors: | ZHANG Xue-mei LI Bao-guo CUI Hui-ying QI Guo-hui |
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| Institution: | ( College of Forestry, Agricultural University of Hebei, Baoding, Hebei 071000, China; General Spreading Station of ForestryTechnique of Hebei Province, Shijiazhuang 050081,China) |
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| Abstract: | Five new apple cultivars,USA No.8,Korin,Matsumoto Nishiki,Huaguan and Nanfangcui,which were introduced into Hebei Province recently,were used as materials in the present study.The primers(P1:5'-TTTACGCAGCAATATCAG-3';P2:5'-ACGTTCGGCCAAATA/CATT-3') were newly designed according to the conserved sequence of amino acid of S-RNase.The self-incompatibility alleles of the cultivars were isolated and identified using PCR-RFLP analysis.Based on the results,the self-incompatibility genotypes of the cultivars were identified as S9S27,S1S9,S9S9,S2S9 and S2S9,respectively. |
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| Keywords: | Apple Self-incompatibility genotype Isolation Identification |
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