| 水稻抗白叶枯病基因Xa23的RFLP标记定位及其STS标记的转化 |
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| 引用本文： | 樊颖伦,陈学伟,王春连,朱立煌,章琦,赵开军. 水稻抗白叶枯病基因Xa23的RFLP标记定位及其STS标记的转化[J]. 作物学报, 2006, 32(6): 931-935 |
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| 作者姓名： | 樊颖伦 陈学伟 王春连 朱立煌 章琦 赵开军 |
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| 作者单位： | 1.中国农业科学院作物科学研究所，农业部作物遗传育种重点实验室，北京100081；2农作物基因资源与基因改良国家重大科学工程，北京100081；3中国科学院遗传与发育生物学研究所，北京100101；4国际水稻研究所中国办事处，北京100081 |
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| 基金项目： | 引进国际先进农业科技计划(948计划);国家科技攻关项目 |
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| 摘 要： | 用水稻抗白叶枯病基因Xa23的近等基因系CBB23与其感病轮回亲本金刚30（JG30）杂交，构建了包含2562个单株的F2作图群体。用水稻白叶枯病广致病菌系P6进行抗性鉴定表明，F2植株抗感分离比严格符合3：1。根据日本水稻基因组计划RGP水稻高密度图谱上的RFLP探针对F2群体中的145个感病单株进行RFLP检测和连锁分析，获得了6个与Xa23紧密连锁的RFLP分子标记。其中RFLP标记C1003A靠着丝粒一侧，与Xa23的遗传图距为0.4cM，为Xa23的图位克隆奠定了重要基础。并将标记C1003A成功地转化为STS标记，为分子标记辅助选择育种（MAS）提供了方便有效的分子标记。
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| 关 键 词： | Xa23 水稻白叶枯病 RFLP标记 STS标记 |
| 收稿时间： | 2005-05-10 |
| 修稿时间： | 2005-11-11 |
Mapping the Rice Bacterial Blight Resistance Gene Xa23 with RFLP Markers and Converting RFLP to STS Marker |
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| FAN Ying-Lun,CHEN Xue-Wei,WANG Chun-Lian,ZHU Li-Huang,ZHANG Qi,ZHAO Kai-Jun. Mapping the Rice Bacterial Blight Resistance Gene Xa23 with RFLP Markers and Converting RFLP to STS Marker[J]. Acta Agronomica Sinica, 2006, 32(6): 931-935 |
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| Authors: | FAN Ying-Lun CHEN Xue-Wei WANG Chun-Lian ZHU Li-Huang ZHANG Qi ZHAO Kai-Jun |
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| Affiliation: | 1.Key Laboratory of Crop Genetics & Breeding, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081;2.National Key facility for Crop Gene Resources and Genetic Improvement, Beijing 100081;3.Institute of Genetics and Developmental Biology , Chinese Academy of Sciences , Beijing,100101;4.IRRI-China Office, Beijing 100081, China |
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| Abstract: | A F_2 population derived from cross of the Xa23 near isogenic lines,JG30 and CBB23,was used for fine mapping of the rice bacterial blight resistance gene Xa23.Inoculation of the F_2 plants with a Xanthomonas oryzae strain P6 showed a 3:1 ratio for the segregation of resistant and susceptible as expected.One hundred and forty-five susceptible plants were selected from the F_2 population for mapping the gene Xa23 and fifteen restriction fragment length polymorphism(RFLP) markers anchored on the rice high-density map. A RFLP marker C1003A was found to be linked to the gene Xa23 by 0.4 cM.This result constitutes an important basis for map-based cloning of the gene Xa23.The RFLP marker C1003A was transformed into sequence-tagged-site(STS) marker,which can be applied for molecular marker-assisted selection(MAS) of Xa23 in rice breeding programs. |
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| Keywords: | Xa23 |
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