Structure of xylan from culms of bamboo grass (Sasa senanensis Rehd.)

Xylan prepared from culms of kumaizasa (Sasa senanensis Rehd.), a representative species of bamboo grass, was hydrolyzed with-xylanase ofStreptomyces olivaceoviridis E-86. Four arabinoxylo-oligosaccharides and two glucuronoxylo-oligosaccharides were isolated from the enzymatic hydrolysate of the xylan by chromatography on a charcoal column, a Dowex 1-x8 column, a Toyo-pearl HW-40S column, and a LiChrospher 100 NH₂ column and on preparative paper chromatography. The results of the structural analyses of the saccharides showed that the isolated oligosaccharides had the structures of 3²--l-arabinofuranosyl-xylobiose, 3²--l-arabinofuranosyl-xylotriose, 3²---d-xylopyranosyl-(1 2)-l-arabinofuranosyl]-xylobiose, 3³---d-xylopyranosyl-(1 2)-l-arabinofuranosyl]-xylotriose, 2³--4-O-methyl-d-glucuronosyl-xylotriose, and 2³--d-glucuronosyl-xylotriose. From the structural analysis of the oligosaccharides derived from the xylan, kumaizasa xylan was concluded to be a kind of arabinoglucuronoxylan having not only stubs of singlel-arabinose and singled-glucuronic acid but also stubs of disaccharide units such as-d-xylopyranosyl-(1 2)-l-arabinofuranose.