1个籼粳杂交F_1花药培养及其影响因素研究

以籼粳杂交(沈农265/R99)F1及其亲本为试验材料,对低温预处理、不同基本培养基、基因型、不同激素配比等影响花药培养效果的因素进行了研究。结果表明:(1)不同低温处理时间沈农265/R99 F1的愈伤诱导率、绿苗分化率和花药培养力差异达到极显著水平,低温预处理7 d的花药培养效果最好。(2)3个基因型的愈伤诱导率、绿苗分化率以及花药培养力差异很大,沈农265/R99适合在SK3培养基上培养,沈农265适合在改良N6培养基上培养,R99适合在M8培养基上培养,培养基与基因型存在互作效应。(3)沈农265/R99F1花药诱导愈伤的最佳的激素配比为2,4-D(2 mg.L-1)、NAA(1mg.L-1)、KT(0.5 mg.L-1)。

F1 Anther Culture from Japonica/indica Hybrid Rice and its Affecting Factors

Effects of low temperature,different culture mediums,genotypes and different hormone mixed rate on anther culture were studied with japonica/indica hybrid F1(Shennong265/R99) as materials.The results showed that the differences of callus induction efficiency,green plantlet differentiation rate and anther culture ability were significant between different low temperature treatments,and low temperature treatment for 7 days had the best effects.The differences of callus induction efficiency,green plantlet differentiation rate and anther culture ability among 3 genotypes were significant and there was interaction between culture mediums and genotypes.The optimal hormone mixed rate was 2,4-D(2 mg·L-1)、NAA(1 mg·L-1) and KT(0.5 mg.L-1).