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| CspB基因植物表达载体的构建及转化玉米自交系的研究 | |
| 引用本文： | 陈丽娟,黄从林,张秀海,吴忠义,杨德光.CspB基因植物表达载体的构建及转化玉米自交系的研究[J].作物杂志,2011(3):70-74. |
| 作者姓名： | 陈丽娟 黄从林 张秀海 吴忠义 杨德光 |
| 作者单位： | 1. 东北农业大学农学院,150030,黑龙江,哈尔滨;北京市农林科学院北京农业生物技术研究中心,100097,北京 2. 北京市农林科学院北京农业生物技术研究中心,100097,北京 3. 东北农业大学农学院,150030,黑龙江,哈尔滨 |
| 基金项目： | 国家转基因重大专项重点课题，北京市科委项目，北京市农林科学院科研能力创新项目 |
| 摘 要： | 利用来自Bacillus subtilis细菌的CspB基因(Gene ID:936224)构建了Ubiquitin启动子驱动的CspB基因植物表达载体PBPC-CspB-bar,以bar基因为抗性筛选标记,通过花粉管通道法将构建的表达载体转化到玉米自交系京501、京517和吉444,通过喷洒除草剂筛选得到60株草丁膦抗性植株,用PCR检测得到48株bar基因阳性植株,将获得的转基因植株进行CspB基因PCR鉴定,获得13株同时整合CspB和bar的转基因株系。 |
| 关 键 词： | 转基因玉米 CspB基因 花粉管通道法 |
Genetic Transformation of CspB in Maize Inbred Lines |
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| Chen Lijuan,Huang Conglin,Zhang Xiuhai,Wu Zhongyi,Yang Deguang.Genetic Transformation of CspB in Maize Inbred Lines[J].Crops,2011(3):70-74. | |
| Authors: | Chen Lijuan Huang Conglin Zhang Xiuhai Wu Zhongyi Yang Deguang |
| Institution: | Chen Lijuan1,2,Huang Conglin2,Zhang Xiuhai2,Wu Zhongyi2,Yang Deguang1(1College of Agricultural,Northeast Agricultural University,Harbin 150030,Heilongjiang,2Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences,100097,Beijing,China) |
| Abstract: | A plant expression vector PBPC-CspB-bar harboring CspB gene driven by corn ubiquitin promoter from the Bacillus subtilis bacteria(Gene ID:936224) was constructed.The bar gene as resistance selection marker,the recombinant plasmid was introduced into maize inbred lines Jing501,Jing517,and Ji444,using pollen-tube-pathway method,and 60 glufosinate-tolerant plants were obtained through herbicide screening.Then 48 transgenic plants were proved by PCR assays for bar gene.To further identify the transgenic plants,... |
| Keywords: | Transgenic corn CspB gene Pollen-tube-pathway method |
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