| 海南槟榔细菌性叶斑病病原鉴定 |
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| 引用本文： | 唐庆华,张世清,牛晓庆,朱辉,余凤玉,宋薇薇,覃伟权.海南槟榔细菌性叶斑病病原鉴定[J].植物病理学报,2014,44(6):700-704. |
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| 作者姓名： | 唐庆华 张世清 牛晓庆 朱辉 余凤玉 宋薇薇 覃伟权 |
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| 作者单位： | 中国热带农业科学院椰子研究所,文昌 571339;中国热带农业科学院热带生物技术研究所,海口 571101 |
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| 基金项目： | 海南省自然基金项目(312041);海南省重大科技项目(ZDZX2013008);中央级公益性科研院所基本科研业务费专项(1630052012016) |
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| 摘 要： |
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Identification of the bacterial leaf spot pathogen of betel palm in Hainan Province |
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| TANG Qing-hua,ZHANG Shi-qing,NIU Xiao-qing,ZHU Hui,YU Feng-yu,SONG Wei-wei,Qin Wei-quan.Identification of the bacterial leaf spot pathogen of betel palm in Hainan Province[J].Acta Phytopathologica Sinica,2014,44(6):700-704. |
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| Authors: | TANG Qing-hua ZHANG Shi-qing NIU Xiao-qing ZHU Hui YU Feng-yu SONG Wei-wei Qin Wei-quan |
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| Institution: | Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences CATAS, Wenchang 571339, China; Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou 571101, China |
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| Abstract: | In 2008, a severe bacterial disease was observed in Wenchang, Hainan Province. Symptoms consisted of small circular to elongated brown lesions surrounded by yellow halos. All isolates, which formed white colonies, were gram-negative and each had a single, polar, sheathed flagellum. Isolates were oxidase and catalase positive, negative for arginine dihydrolase, gelatin hydrolysis and starch hydrolysis, and acid production from levan. Sequences of the 16S rDNA gene of the isolates shared 98% sequence identity with that of Burkholderia andropogonis strain LMG2129 (GenBank accession No. NR118985). Pathogenicity tests were conducted and fulfilled Koch's postulates, indicating that these isolates are causative agent of the disease. The results led to the conclusion that the causal pathogen of betel palm bacterial leaf spot was B. andropogonis. It is the first report of B. andropogonis causing bacterial leaf spot of betel palm in mainland China. |
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| Keywords: | bacterial leaf spot of betel palm Burkholderia andropogonis molecular identification |
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