茯苓菌菌丝的核相及染色技术研究

以茯苓菌为材料,采用Giemsa、番红O-KOH、结晶紫、石炭酸碱性复红以及Hoechest 33258 5种染料对茯苓菌丝进行染色,研究其核相及锁状联合现象。结果显示:Giemsa染色效果最好,番红O-KOH和Hoechest 33258次之,结晶紫和石炭酸碱性复红最差。在普通光学显微镜以及荧光显微镜下,采用Giemsa和Hoechest 33258染色可见茯苓菌丝为多核菌丝体,但难以辨别茯苓是否具有锁状联合。

Research on Nucleus Distribution of Poria cocos and its Staining Technique

Poria cocos originated from different regions being taken as materials,the mycelia growth situation,nucleus distribution and clamp connection were observed through the application of staining techniques such as Giemsa,Safranin O-KOH,Crystal violet,Basic carbolfuchsin and Hoechest 33258 in this study.The results showed that Giemsa and Hoechest 33258 staining methods exhibited the best staining efficiency,better one was Safranin O-KOH staining and last one was Crystal violet and Bacic carbolfuchsin staining.Multinucleate of Poria cocos could be observed under the fluorochromy microscope via Hoechest 33258 staining,but it was difficult to observe the existence of clamp connection.Under the light microscope,the septum and branching can be clearly observed in all above staining techniques,but the multinucleate of the Poria cocos could be clearly seen only in Giemsa staining.At the same time,the effectiveness of the same staining technique in different times was compared in this experiment.The result showed that Giemsa and Hoechest 33258 staining were the best in three minutes,Safranin O-KOH method was the best in five minutes staining and Crystal violet staining only needed one minute,but Bacic carbolfuchsin staining needed nine minutes.