| 转sbk和sck抗虫基因水稻的获得 |
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| 引用本文： | 张启军,吕川根,夏士健,宗寿余,漆庆明,虞德容,孙永华. 转sbk和sck抗虫基因水稻的获得[J]. 分子植物育种, 2008, 6(1): 49-52 |
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| 作者姓名： | 张启军 吕川根 夏士健 宗寿余 漆庆明 虞德容 孙永华 |
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| 作者单位： | 江苏省农业科学院粮食作物研究所,国家水稻改良中心南京分中心,南京,210014 |
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| 基金项目： | 国家高技术研究发展计划(863计划) , 江苏省农业科学院资助项目 |
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| 摘 要： | 转入多个抗虫基因是解决水稻抗虫的一条有效途径.本研究采用根癌农杆菌介导法将2个抗虫基因(sbk和sck)同时转入3个水稻品种中,通过一系列的分子检测(PCR和RT-PCR)分析,这2个基因已经成功转入3个水稻品种中并得到了表达;同时还获得1株不含抗生素筛选标记基因(hpt)和抗虫sbk基因,却含抗虫sck基因的转基因植株,命名为YJ27-5sck.用该植株作为供体,通过杂交将sck抗虫基因转育到另外5个水稻品种中且同样得到了表达,获得了不含筛选标记基因的转sck基因阳性植株.
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| 关 键 词： | 水稻 抗虫基因 转基因植株 基因表达 抗虫基因 基因水稻 Genes Plants 阳性植株 转育 杂交 命名 基因植株 标记基因 筛选 抗生素 表达 分析 分子检测 水稻品种 农杆菌介导法 根癌 研究 |
Obtaining Transgenic Rice Plants Harboring sbk and sck Insecticidal Genes |
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| Zhang Qijun,Lv Chuangen,Xia Shijian,Zong Shouyu,Qi Qingming,Yu Derong,Sun Yonghua. Obtaining Transgenic Rice Plants Harboring sbk and sck Insecticidal Genes[J]. Molecular Plant Breeding, 2008, 6(1): 49-52 |
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| Authors: | Zhang Qijun Lv Chuangen Xia Shijian Zong Shouyu Qi Qingming Yu Derong Sun Yonghua |
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| Affiliation: | Zhang Qijun Lv Chuangen Xia Shijian Zong Shouyu Qi Qingming Yu Derong Sun Yonghua Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing Branch of National Rice Improvement Center,Nanjing,210014 |
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| Abstract: | It might be one of the effective ways to solve the problems of rice insect harm by introducing multigenes into rice. In this research, two insecticidal genes (sbk and sck) have been transferred into three rice varieties mediated by Agrobacterium tumerfaciens approach, the results of PCR identifications indicated that the two genes have been integrated into the genomes of tested three rice varieties and the targeted genes would be successfully expressed. And also we distinguished a transgenic individual whic... |
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| Keywords: | Rice Insecticidal gene Transgenic plant Gene expressing |
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