β-氨基丁酸和茄青枯菌hrp-突变体协同防治辣椒疫病研究

将具有不同作用机理的植物化学诱抗剂β-氨基丁酸(BABA)与无致病力的茄青枯菌hrp-突变体菌株联合使用,研究不同配比对辣椒疫病的协同防治效果和使用技术。结果表明,BABA与hrp-菌株按一定配比混合后于接种前使用一次,与单剂相比对辣椒疫病均有不同程度的增效作用。其中0.08%BABA+hrp-菌株1×10~7 cfu/m L、0.1%BABA+hrp-菌株1×10~6 cfu/m L和0.1%BABA+hrp-菌株1×10~5 cfu/m L等3种配比组合控制辣椒疫病的效果均在80%以上;与BABA单独使用相比,增效作用超过23%;以0.1%BABA+hrp-菌株1×10~6 cfu/m L配比效果最佳,其增效达到43%。适当增加使用次数后,防治效果及持效期均得到进一步提高。本研究为探索多种生防措施的协调和配合应用开辟了一条新途径。

Synergistic Efficacy of BABA and Hrp-mutant of Ralstonia solanacearum against Pepper Blight

The synergistic efficacy and application technique of the mixture of β-aminobutyric acid (BABA) and avirulent hrp-mutant ofRalstonia solanacearum against pepper blight with different proportion were determined.The results showed that pretreatment of the combined use of BABA and hrp-mutant before inoculation obviously increased the control effects on pepper blight compared with single agent.Among six combinations of BABA and hrp-mutant,pretreatment with 0.08％ BABA+hrp-strain 1 × 107cfu/mL,0.1％ BABA+hrp-strain 1 × 106 cfu/mL,or 0.1％ BABA+hrp-strain 1 × 105 cfu/mL displayed the increase of control effect over 80％,and enhanced the synergy over 23％,compared with the single treatment with BABA.Moreover,pretreatment with the combined with 0.1％ BABA+hrp-strain 1 × 106 cfu/mL showed the best efficacy with the synergy of 43％.The control effect and duration of the efficacy against pepper blight were further improved by increasing the frequency of application treatment.This work explored a new way for revealing the coordination application of multiple biocontrol measures.