播娘蒿幼蕾和花序轴的组织培养和再生植株研究

播娘蒿幼小花蕾在附加有１５ｍｇ／ＬＮＡＡ、０５ｍｇ／Ｌ６－ＢＡ和０１ｍｇ／ＬＫＴ的ＭＳ培养基中，培养两周即形成０５ｃｍ２大小的愈伤组织块，出愈率达８３％。继续培养两周转至附加有２ｍｇ／Ｌ６－ＢＡ、０５ｍｇ／ＬＫＴ和０２ｍｇ／ＬＮＡＡ的ＭＳ分化培养基上，２～３周后出现绿色芽点，继续培养２周即可得到分化小芽。播娘蒿花序轴在附加有２～６ｍｇ／Ｌ６－ＢＡ、０５ｍｇ／ＬＮＡＡ的ＭＳ培养基中，培养５～６周也都能分化出芽，但最佳培养基为４ｍｇ／Ｌ６－ＢＡ、０５ｍｇ／ＬＮＡＡ。分化小芽转至附加０５ｍｇ／ＬＮＡＡ或０５ｍｇ／ＬＩＢＡ的１／２ＭＳ生根培养基上培养２～３周均能形成完整植株。

Studies on tissue culture and plantlet regeneration of tiny buds and floral axes of Descurainia sophia

Tiny young buds of D.sophia were cultured in the medium of 1.5mg/L NAA 0.5mg/L6-BA 0.1mg/L KT MS for two weeks and their callus were formed.The frequency reached about 83%.Two weeks later small buds were transplanted onto differentiation medium of 2mg/L6-BA 0.5mg/L KT 0.2mg/L NAA MS.They were cultured for two weeks more.Then green spots appeared.Two weeks later,differentiated shoots formed.Floral axes in the medium of 2-6mg/L 6-BA 0.5mg/L NAA MS for 5-6 weeks and then their shoots were formed among these kinds of medium,the best one is the one with 4mg/L 6-BA.The differentiated young shoots were transplanted onto the rooting medium of 05mg/L NAA or 05mg/L IBA 1/2MS. 2-3 weeks later,complete plantlets were regenerated.