| 高山被孢霉挥发性次生代谢产物分析 |
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| 引用本文： | 刘欣,马小琛,黄和,于文涛,纪晓俊,彭超,高振.高山被孢霉挥发性次生代谢产物分析[J].农业科学与技术,2009,10(4):12-14,21. |
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| 作者姓名： | 刘欣 马小琛 黄和 于文涛 纪晓俊 彭超 高振 |
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| 作者单位： | 刘欣,马小琛,于文涛,纪晓俊,彭超,高振(南京工业大学生物与制药工程学院,江苏南京,210009);黄和(南京工业大学生物与制药工程学院,江苏南京,210009;江苏省工业生物技术创新中心,江苏南京,211816) |
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| 基金项目： | the National Natural Science Foundation of China，the National Basic Research Program (also called 973 Program)，the Six Projects Sponsoring Talent Summits of Jiangsu Province.国家自然科学基金，973计划，江苏省六大人才高峰资助项目 |
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| 摘 要： | 1材料与方法
1．1试剂酵母粉为英国OXOID公司生产；葡萄糖、KH2PO4、MgSO4·7H2O均为国产分析纯。
1．2仪器Thermo Finnigan TRACE DSQ型气相色谱-质谱联用仪；SUPELCO公司的SPME Fiber Assortment Kit #1固相微萃取头。
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| 关 键 词： | 高山被孢霉 产物分析 次生代谢 气相色谱-质谱联用仪 挥发性 KH2PO4 MgSO4 TRACE |
Analysis for the Volatile Secondary Metabolites of Mortierella alpina |
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| Institution: | LIU Xin MA Xiao-chen HUANG He YU Wen-tao JI Xiao-jun PENG Chao GAg Zhen( 1. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009; 2. Jiangsu Provincial Innovation Center for Industrial Biotechnology, Nanjing 211816) |
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| Abstract: | Objective] The study was to investigate the volatile components of secondary metabolites from M. alpine producing arachidonic acid and explore the changes in its metabolic pathway. Method] The air above M. alpine broth was extracted by solid-phase microextraction（SPME） during the post-exponential phase of growth and analyzed by GC-MS. Result] 13 compounds were identified, 12 of which were sesquiterpenes with C15H24 formula and accounting for 99.62% of the complete compounds. Thujopsene-（ 12）, α-Guaiene and Aristolene were three most sesquiterpenes accounting for 10.66%, 33.69% and 34.85% of total content respectively. It can be sufficiently certified that sesquiterpene metabolic pathway existing in M. alpine. Coclusion] Metabolic flux of sesquiterpene pathway increased to improve its mass accumulation, because one or several key enzyme genes mutation in isoprene or sesquiterpene pathway enhanced their activities during induction of mutation from initial strain. |
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| Keywords: | Mortierella alpina SPME Sesquiterpene Arachidonic acid |
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