5种切花菊内生真菌的分离鉴定与分布情况研究

研究了5个常见切花菊品种内生真菌的分离鉴定与分布情况,以寻找切花菊自身抗性真菌并为今后开展生物防治提供基础资料。找到了切花菊各部位材料的表面灭菌处理最优方法,发现切花菊内生真菌在分离中出现幼嫩部位内生真菌分离数量明显高于成熟部位,并且根分布最多,叶次之,茎最少。5个切花菊的内生真菌中,镰刀菌属(Fusarium)和枝孢属(Cladosporium)为优势菌群。5个切花菊品种内生真菌的分布存在一定相似度,但不同品种还是存在自己特有的内生真菌,说明切花菊的内生真菌分布有一定多样性。

Research on Isolation,Identification and Distribution of Endophytic Fungi from Five Cut Chrysanthemums

The isolation, identification and distribution of endophytic fungi from five common cut chrysanthemum cultivars were studied to find out their own resistant fungi and provide fundamental data for the future studies on biological control.The optimal method for surface sterilization treatment of various parts of cut chrysanthemum materials was found.It was found that the quantity of isolated endophytic fungi from the tender part of cut chrysanthemums was obviously higher than that from the mature part, and the distribution of endophytic fungi in different parts showed the following order：root＆gt;leaf＆gt;stem.Among endophytic fungi from five cut chrysanthemums, Fusarium and Cladosporium were dominant fungal community.Five cut chrysanthemum cultivars were somewhat similar in the distribution of endophytic fungi, but different cultivars also had their own unique endophytic fungi, indicating that the distribution of endophytic fungi in cut chrysanthemums had a certain diversity.