红果肉海棠资源亲缘关系的AFLP分析

利用AFLP分子标记技术,以部分苹果栽培种和苹果属野生种为参照,对23种红果肉海棠的亲缘关系进行了研究。在64对引物中筛选出4对进行选择性扩增,共得到94条有效谱带,其中多态谱带92条,多态谱带占97.9%。UPGMA聚类分析显示,30份供试材料的相似系数为0.63~0.95,在相似系数为0.65时,样品可划分为4组,A组中包括6种欧美海棠高原之火、路易莎、秀场、道格、钻石、红巴伦以及粉女士;B组中包括霍巴、丽丝、雷蒙、玛瑙、王族、粉芽、红丽、火焰、完美紫叶、印第安夏天等红果肉杂交种以及野生种新疆红肉苹果、花红和山荆子;C组包括印第安魔力、朱砂、三叶海棠、珠眉海棠、绚丽、凯尔斯、撒氏;D组包括新疆野苹果、富士苹果、嘎啦苹果。从分子水平揭示了它们之间的亲缘关系,为红果肉苹果杂交育种的亲本选配提供了理论依据。

Relationship of Red-flashed Crabapples by AFLP Analysis

As a reference to part cultivars and wild crabapple species, amplified fragment length polymorphism (AFLP) analysis was used to reveal the relationships of red-flashed crabapples.Four pairs of primer combinations selected from 64 combina-tions produced 94 legible loci with 92 polymorphic.The polymorphism rate was 97.9%.By UPGMA clustering analysis, the genetic similarity coefficient among 30 samples varied in 0.63-0.95.Four groups of taxa were assigned with the genetic similarity coefficient at 0.65.Group A contained M.‘Prairifire’ , M.‘Louisa’ , M.‘showtime’ , M.‘Dolgo’ , M.‘Spar-kler’, M.‘Red Barron’and M.×domestica‘Pink Lady’; Group B contained red fleshed hybrid like M.‘Hopa’, M.‘Liset’, M.‘Lemoinei’, M.‘Agate’, M.‘Royalty’, M.‘Pink spires’, M.‘Red Splender’, M.‘Flame’, M.‘Per-fect Purple’, M.‘Indian Summer’ and wild species of M.sieversii.var.‘Niedzwetzkyana’, M.asiatica.and M.bacca-ta.;Group C contained M.‘Indian Magic’, M.‘Cinnabar’, M.sieboldii, M.×zumi, M.‘Radiant’, M.‘Kelsey’ and M.‘Sargentii’;Group D contained M.Sieversii, M.×domestica ‘Fuji’and M.×domestica ‘Gala’.The genetic relation-ship between them could be used for parent selection in red-flashed crabapples breeding.