| 西南区多花黑麦草品种同工酶指纹图谱的构建及遗传背景分析 |
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| 引用本文： | 黄琳凯,张新全,马啸,刘明秀,曾琨.西南区多花黑麦草品种同工酶指纹图谱的构建及遗传背景分析[J].安徽农业科学,2006,34(3):426-427,443. |
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| 作者姓名： | 黄琳凯 张新全 马啸 刘明秀 曾琨 |
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| 作者单位： | 四川农业大学动物科技学院,四川,雅安,625014;四川农业大学动物科技学院,四川,雅安,625014;四川农业大学动物科技学院,四川,雅安,625014;四川农业大学动物科技学院,四川,雅安,625014;四川农业大学动物科技学院,四川,雅安,625014 |
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| 基金项目： | 四川省科技攻关项目
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科技部星火计划 |
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| 摘 要： | 采用聚丙烯酰胺凝胶电泳的方法,对西南区主栽6个多花黑麦草品种进行了酯酶(EST)和过氧化物酶(POD)同工酶检测。通过酯酶检测到5种EST酶谱谱型,可以把4个品种区分开。过氧化物酶检测到4种POD酶谱谱型,可以把3个品种区分开。综合以上2种酶谱谱型可以鉴定供试的6个品种。6个多花黑麦草之间的相似系数0.615~0.941,品种间平均相似系数为0.738 8。
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| 关 键 词： | 多花黑麦草 同工酶 品种鉴定 聚类分析 |
| 文章编号： | 0517-6611（2006）03-0426-02 |
| 收稿时间： | 2005-10-14 |
| 修稿时间： | 2005-10-14 |
Isozyme Fingerprint of Six Varieties of Lolium multifolium in South-west China and Their Genetic Background |
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| HUANG Lin-kai et al.Isozyme Fingerprint of Six Varieties of Lolium multifolium in South-west China and Their Genetic Background[J].Journal of Anhui Agricultural Sciences,2006,34(3):426-427,443. |
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| Authors: | HUANG Lin-kai |
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| Institution: | College of Animal Science and Technology, Sichuan Agricultural University, Yaan, Siehuan 625014 |
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| Abstract: | The esterase(EST) isozyme and peroxidase(POD) isozyme of Annual ryegrass in south-west China has been studied with polyarcylamide gel electrophoresis. EST yielded 5 different isozume phenotypes and five varieties were discriminated by this enzyme alone.POD produced 4 different isozyme phenotypes and 4 varieties were separated.When both enzyme systems were taken together,6 varieties were uniquely discriminated.The similarity coefficient among 6 varieties of annual ryegrass ranged from 0.615 to 0.941,with an average of 0.738?8. |
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| Keywords: | Annual ryegrass Isozyme Variety identification Cluster analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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