磁化水处理对桑树硬枝扦插生根生理的影响

以非磁化自来水为对照（ck），以0.06，0.14和0.22 T等3种磁感应强度磁化水处理器处理自来水对桑树品种‘鲁插1号’Morus alba ‘Lucha-1’扦插苗进行喷淋。通过测定插条基部可溶性蛋白质和可溶性糖质量分数、内源激素质量分数、生根数量、根系形态及根系矿物质元素质量分数，分析不同磁感应强度对‘鲁插1号’桑树硬枝扦插生根生理特性的影响。结果表明:①磁化水处理使扦插苗根系生物量、总根长、总表面积、总体积、平均直径及根系形态特征值等显著提高（P < 0.05）；扦插苗根系氮、钾、锌质量分数均显著高于对照（P < 0.05），铁质量分数显著降低（P < 0.05）。②磁化水处理显著提高桑树扦插苗可溶性糖和可溶性蛋白质质量分数（P < 0.05），磁感应强度越高效果越明显，显著增加了吲哚丁酸（IBA）和吲哚乙酸（IAA）质量分数（P < 0.05），显著降低了脱落酸（ABA）和玉米素（ZT）质量分数（P < 0.05），显著提升了扦插苗生根数量、根系活力水平和生根率（P < 0.05）。综上所述，磁化水处理不仅影响桑树品种‘鲁插1号’的扦插苗不定根形成，且随着喷淋时间的延长，对根系生长及发育有明显的促进作用。

Physiological characteristics of hardwood cuttings from mulberry trees with magnetic field conditions

To explore the influence of magnetized water (MW) on roots architecture of Morus alba, magnetizers of a PP-25-ADS with different magnetic intensities (0.06, 0.14 and 0.22 T) were used to irrigate one-year-old hardwood mulberry cuttings, and the non-magnetized tap (0 T) was set as the control (ck). The contents of soluble proteins, soluble sugars and endogenous hormones, parameters of root morphology, rooting rates, and rooting activities were measured. A randomized block experiment design was used with 5 replicates of each treatment. After irrigating with different magnetic field strength for 10 days, Ten cuttings with uniform growth were selected for every ten days, and there were three replications in per treatment. The cuttings were rinsed with distilled water and used to measure the important physiological and biochemical indexes. Enzyme-linked immunsorbent assay (ELISA) was used to determine the phytohormones level (IAA, IBA, ZT and ABA); phenol method was adopted to determine the content of soluble sugar; coomassie brilliant blue staining was applied to measure the content of soluble protein, and the WinRHIZO system was adapted to analyse the root morphology. The results showed that (1) Biomass, total length, total surface area, volume, and average root diameter, as well as the morphological characteristics were significantly improved (P < 0.05) by MW treatment. Contents of the mineral elements nitrogen (N), potassium (K), and zinc (Zn) were remarkably increased (P < 0.05) in MW compared to ck, but the iron (Fe) significantly decreased (P < 0.05). Phosphorus (P), manganese (Mn), and copper (Cu) was no marked differences (P > 0.05). (2) The levels of soluble sugar, soluble protein, indole butyric acid (IBA), and indoleacetic acid (IAA) were significantly promoted by MW (P < 0.05); whereas, levels of abscisic acid (ABA) and zeatin (ZT) were markedly decreased (P < 0.05). Moreover, root number, root vigor, and rooting rates were a bit higher in the treament than that of in the control (P < 0.05). In conclusion, MW could positively influence the formation of adventitious roots with mulberry seedlings and could improve the growth and development of roots with prolonged spraying time.