洛阳、吉林生态区谷子抗倒伏性的全基因组关联分析

倒伏是影响谷子产量和品质的重要因素,为揭示谷子抗倒伏性的遗传机制,在洛阳、吉林两地调查了98份谷子品种的倒伏性,基于重测序技术开展SNP标记与抗倒伏性状的全基因组关联分析。研究结果表明:98份谷子品种倒伏率在洛阳、吉林两地均表现为无倒伏品种数>轻微倒伏品种数>严重倒伏品种数。基因组重测序开发了4 482 208个SNP标记,群体结构分析将98份谷子材料划分为3个亚群,LD分析发现谷子基因组连锁不平衡衰退距离为47.5 kb。全基因组关联分析在洛阳、吉林分别检测到764个和24个与谷子抗倒伏性关联的SNP位点,在6号染色体共同检测到一个LRR受体类丝氨酸/苏氨酸蛋白激酶基因(LOC101776261),此外在6号、8号染色体还检测到位点不同但功能接近的细胞壁关联受体激酶基因(LOC105914550、LOC101786429、LOC105913474、LOC101766879),还有一些基因,如细胞色素P450蛋白、UDP糖基转移酶、驱动蛋白在两地不同染色体上检测到,推测这些基因可能与谷子的抗倒伏性相关。谷子抗倒伏性是由多基因控制的复杂数量性状,其中蛋白激酶类基因可能在谷子倒伏抗性中起到重要作用。

Genome-wide association analysis of lodging resistance of millet in Luoyang and Jilin ecological regions

Lodging is an important factor affecting the yield and quality of millet. In order to uncover the genetic mechanism of lodging resistance in foxtail millet, 98 foxtail millet cultivars were selected to investigate lodging rate in Luoyang and Jilin, further re-sequencing was performed for the association analysis between SNPs and lodging resistance. The results showed that lodging rate of 98 foxtail millet cultivars gave same distribution law in Luoyang and Jilin: number of cultivars without lodging>number of cultivars with fewer lodging>number of cultivars with quite a few lodging. 4 482 208 SNP markers were developed by re-sequencing and the 98 foxtail millet cultivars were divided into three groups by population structure analysis. LD analysis found that linkage disequilibrium decline distance of foxtail millet genome was 47.5 kb. 764 and 24 SNP sites associated with lodging resistance were detected in Luoyang and Jilin respectively by genome-wide association analysis. A candidate gene coding LRR receptor-like serine/threonine-protein kinase (LOC101776261) on Chr6 was detected both in Luoyang and Jilin. Furthermore, some wall-associated receptor kinase genes(LOC105914550,LOC101786429,LOC105913474,LOC101766879) that were function-related but lied in different loci were detected on Chr6 and Chr8 in Luoyang and Jilin. Some genes such as cytochrome P450 protein, UDP-glycosyltransferase and kinesin protein were detected on different chromosomes in Luoyang and Jilin. All of candidate genes above may be related to lodging resistance of foxtail millet. Lodging resistance was a complicated quantitative character that controlled by multiple genes in foxtail millet and the protein kinase genes may play important role in lodging resistance of foxtail millet.