海南黑山羊生长激素（GH）基因单核苷酸多态性分析

利用PCR-SSCP与测序相结合，分析海南黑山羊GH基因第2外显子exon2的多态性，SSCP结果出现3种基因型，分别定义为AA、AB、BB；3种基因型频率分别为28．3％、63．0％、8．7％，A、B等位基因频率分别为59．8％和40．2％，以A等位基因为优势基因，经Hardy-Weinberg平衡卡方适合性检验结果表明，exon2在此群体中处于Hardy-Weinberg不平衡状态（X2=0．000，P〈0．01），该位点群体遗传结构及多态指标分析结果显示，该位点表现为中度多态。AA和BB基因型进行测序结果发现，exon2第11位点发生G→C突变，将核苷酸序列翻译成氨基酸序列进行同源性比较，结果exon2第11位G→C突变位点引起编码的氨基酸改变，所编码的氨基酸由原来的精氨酸变为脯氨酸。

Single Nucleotide Polymorphism Analysis of Hormone (GH) Growth Gene in Hainan Black Goat

The polymorphism of Hainan black goat GH gene exon 2 was analysed by PCR-SSCP and sequencing.The SSCP result showed that 3 genotypes were discovered and defined as AA,AB,BB with their frequencies being 28.3%,63.0%,8.7%,respectively,and the A,B allele frequencies being 59.8% and 40.2%.The Hardy-Weinberg balance for Chi-square test with allele gene of B as the dominance shows that the exon2 was imbalanced in the group at the Hardy-Weinberg(X2=0.000,P<0.01).Population genetic structure and polymorphism analysi...